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Analysis of vernix caseosa lipids by chromatografic methods and mass spectrometry
Míková, Radka
(EN) Vernix caseosa is a white creamy substance that covers the skin of a newborn. It is produced during the third trimester by the skin of the baby and remains there until the age of one or even two weeks. It is uniquely human. In utero, vernix protects the skin from maceration, during the birth it serves as a lubricant and after the delivery it protects the baby against infection and regulates the temperature. As vernix is produced in third trimester, prematurely born infants lack it and this may lead to, among other things, suffering from desiccation and therefore heat loss. It is important to study it thoroughly and to find a suitable substitute of vernix for the preterm infants. Vernix consists of lipids, proteins and 80 % water. This project is aimed at the lipids. Vernix is composed of 10 % of lipids. Basic analytical methods of pocessing vernix were searched. The methods of isolation, separation and transesterification have been optimized for the lipids. For separation, thin-layer chromatography has been chosen. The method of the lipid analysis of intact molecules by MALDI-TOF MS has been optimized for these lipids. The results were confirmed using fragmentation spectra and transesterification. Esterified lipids were measured by gas chromatography coupled with mass spectrometry detection....
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Analysis of vernix caseosa lipids by chromatografic methods and mass spectrometry
Míková, Radka ; Cvačka, Josef (advisor) ; Jelínek, Ivan (referee) ; Tůma, Petr (referee)
(EN) Vernix caseosa is a white creamy substance that covers the skin of a newborn. It is produced during the third trimester by the skin of the baby and remains there until the age of one or even two weeks. It is uniquely human. In utero, vernix protects the skin from maceration, during the birth it serves as a lubricant and after the delivery it protects the baby against infection and regulates the temperature. As vernix is produced in third trimester, prematurely born infants lack it and this may lead to, among other things, suffering from desiccation and therefore heat loss. It is important to study it thoroughly and to find a suitable substitute of vernix for the preterm infants. Vernix consists of lipids, proteins and 80 % water. This project is aimed at the lipids. Vernix is composed of 10 % of lipids. Basic analytical methods of pocessing vernix were searched. The methods of isolation, separation and transesterification have been optimized for the lipids. For separation, thin-layer chromatography has been chosen. The method of the lipid analysis of intact molecules by MALDI-TOF MS has been optimized for these lipids. The results were confirmed using fragmentation spectra and transesterification. Esterified lipids were measured by gas chromatography coupled with mass spectrometry detection....
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Analysis of vernix caseosa lipids by chromatografic methods and mass spectrometry
Míková, Radka
(EN) Vernix caseosa is a white creamy substance that covers the skin of a newborn. It is produced during the third trimester by the skin of the baby and remains there until the age of one or even two weeks. It is uniquely human. In utero, vernix protects the skin from maceration, during the birth it serves as a lubricant and after the delivery it protects the baby against infection and regulates the temperature. As vernix is produced in third trimester, prematurely born infants lack it and this may lead to, among other things, suffering from desiccation and therefore heat loss. It is important to study it thoroughly and to find a suitable substitute of vernix for the preterm infants. Vernix consists of lipids, proteins and 80 % water. This project is aimed at the lipids. Vernix is composed of 10 % of lipids. Basic analytical methods of pocessing vernix were searched. The methods of isolation, separation and transesterification have been optimized for the lipids. For separation, thin-layer chromatography has been chosen. The method of the lipid analysis of intact molecules by MALDI-TOF MS has been optimized for these lipids. The results were confirmed using fragmentation spectra and transesterification. Esterified lipids were measured by gas chromatography coupled with mass spectrometry detection....
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Psychological aspects of pregnancy risk
MIKOVÁ, Radka
The thesis focuses on the psychological aspects of risk pregnancy perception. The primary objective of the thesis is to deal with the various aspects of the risk pregnancy experience and analyze the importance of psychological support. The work is divided into theoretical and empirical part. The theoretical part explains the terms of physiological pregnancy and risk pregnancy in the broader context and describes the basic psychological concepts related to the topic. In the empirical part specified and justified hypothesis are presented together with the selected way of measuring data questionnaire method, data processing and analysis. There is presented a detailed analysis of responses from the survey including interpretation of the results. The conclusion is a summary of all the previous parts. There is addressed verification of hypotheses and objectives of the thesis. The problems in the process of creation of the thesis are outlined and benefits and utility work in practice are explained. The research results showed the importance and significance of psychological support during risk pregnancy. The thesis proved that emotional support can be found in multiple sources, but the greatest support for risk pregnant women is represented by husband or partner. It was also showed that there are many sources of information and risk pregnant women are interested in them. Understanding the whole situation is crucial for the acceptance of all restrictions and treatment recommendations that relate to the situation. And all these factors - psychological support, information and understanding of the situation - will ultimately have a positive impact on the overall psychological state of risk pregnant women.
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Social environement as a factor influencing availability of information about pregnancy and childbirth
MIKOVÁ, Radka
This bachelor work investigates the problems of influence of social environment on the awareness of pregnancy and birth. The primary aim of this work is to map out the demand for information, its availability and the most commonly used resources of information. The next aim is to analyse the obtained data from the perspective of age, education and marital status. The results of the research showed a significant influence of social environment on awareness of pregnancy and birth. The relationship of age, education and marital status on the level of awareness has been proven. Information related to child birth is greatly available. This is to a great extent also thanks to media that balance out the limited demand for information in certain age and education categories.
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