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The Determination and Comparison of the Electromigration Properties of Markers for Isoelectric Focusing
Lorinčíková, Kateřina ; Gaš, Bohuslav (advisor) ; Kašička, Václav (referee)
The dependencies of electrophoretic mobility on pH were measured for a set of 14 markers used for isoelectric focusing that were developed by the group of Šlais and that are based on substitutions on the nitrophenol core, and for a kit consisting of 5 pI markers developed by Shimura, which have an oligopeptide structure. The dissociation constants and limiting electrophoretic mobilities of these compounds were obtained from the dependencies with the use of the program AnglerFish. The isoelectric point values of the compounds were consequently calculated using the obtained data. A comparison of the obtained pI values with the values that have been declared in literature, albeit gained by different analytical methods, has been made. Key Words capillary zone electrophoresis, isoelectric focusing, pI markers, isoelectric point, thermodynamic dissociation constant, limiting ionic mobility
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Zero Ionic Strength Corrections of pKA Constants and Electrophoretic Mobilities of Weak Electrolytes
Lorinčíková, Kateřina ; Gaš, Bohuslav (advisor) ; Sobotníková, Jana (referee)
The dependencies of the electrophoretic mobility of histidine on pH were measured in two sets of buffers at 20mM ionic strength. The parameters, which were obtained from these curves, were subsequently corrected to zero ionic strength using an older approach, as well as using the newly developed program AnglerFish. The effect of the ionic atmosphere on the pKA and mobility is shown, as well as the shape of the relevant corrections, which allow the determination of the pKA and mobility at infinite dilution. A new compound, imatinib, which is a quadruple positively charged nitrogenous heterocyclic compound, was also measured, and its pKA constants and mobilities were likewise determined. Key Words capillary zone electrophoresis, ionic strength, limiting mobility, pKA, zero ionic strength corrections
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