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Selective regulation of presynaptic receptors by SGIP1
Durydivka, Oleh ; Blahoš, Jaroslav (advisor) ; Bondar, Alexey (referee) ; Lazar, Josef (referee)
Selective regulation of presynaptic receptors by SGIP1 Abstract Cannabinoid receptor 1 (CB1R) is involved in a plethora of physiological processes, such as memory formation, motor coordination, anxiety, pain perception, and immune response. The properties of many minor cannabinoid receptor ligands remain unknown. The activity of CB1R is regulated by Src homology 3-domain growth factor receptor-bound 2-like endophilin interacting protein 1 (SGIP1). Several splice variants of SGIP1 have been described in the literature, but their specific functional roles are unknown. SGIP1 inhibits CB1R internalization and enhances β-arrestin and G protein-coupled receptor kinase 3 (GRK3) interactions with the receptor. In mice, deletion of Sgip1 results in altered mood-related behavior, decreased anxiety- like behavior, and decreased acute nociception. In this work, we tested the effect of Sgip1 deletion on chronic nociception. We further explored the pattern of alternative splicing of Sgip1 in the brain. In addition, we tested the effect of the minor cannabinoid hexahydrocannabinol (HHC) on CB1R signaling. We found that Sgip1 deletion results in an increase in chronic nociception in male but not in female mice. We detected 15 Sgip1 splice variants in the mouse brain. The Sgip1 exons that undergo alternative splicing encode...
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Methods of Preparation and Characterization of Experimental Field-Emission Cathodes
Knápek, Alexandr ; Luňáček, Jiří (referee) ; Lazar, Josef (referee) ; Grmela, Lubomír (advisor)
Téma doktorské práce se zabývá přípravou a popisem katod na bázi autoemise, jenž představují kvalitní a levný elektronový zdroj pro zařízení pracující s fokusovaným elektronovým svazkem. Pro přípravu kompozitní autoemisní katody byla využita elektrochemická metoda výroby. Kompozitní struktura katody zlepšuje proudovou stabilitu ve srovnání s čistě autoemisními katodami na bázi wolframu. Na základě charakterizace katody, jenž byla nově provedena metodou šumové spektroskopie, byla implementována technologická zlepšení stávající výroby. Metoda šumové spektroskopie je založena na analýze emisního proudu v časové a kmitočtové rovině, ale především poskytuje informace o nosiči náboje, o jeho pohyblivosti a dále o životnosti katody. Výsledky experimentální části byly rozšířeny teoretickými simulacemi, vedoucími k návrhu metodiky charakterizace vylepšené autoemisní katody.
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Noise, Transport and Structural Properties of High Energy Radiation Detectors Based on CdTe
Šik, Ondřej ; Lazar, Josef (referee) ; Navrátil, Vladislav (referee) ; Grmela, Lubomír (advisor)
Poptávka ze strany vesmírného výzkumu, zdravotnictví a bezpečnostního průmyslu způsobila v posledních letech zvýšený zájem o vývoj materiálů pro detekci a zobrazování vysokoenergetického záření. CdTe a jeho slitina CdZnTe. jsou polovodiče umožnují detekci záření o energiích v rozsahu 10 keV až 500 keV. Šířka zakázaného pásma u CdTe / CdZnTe je 1.46 -1.6 eV, což umožňuje produkci krystalů o vysoké rezistivitě (10^10-10^11 cm), která je dostačující pro použití CdTe / CdZnTe při pokojové teplotě. V mé práci byly zkoumány detektory CdTe/CdZnTe v různých stádiích jejich poruchovosti. Byly použity velmi kvalitní spektroskopické detektory, materiál s nižší rezistivitou a výraznou polarizací, detektory s asymetrií elektrických parametrů kontaktů a teplotně degenerované vzorky. Z výsledků analýzy nízkofrekvenčního šumu je patrný obecný závěr, že zvýšená koncentrace defektů způsobí změnu povahy původně monotónního spektra typu 1/f na spektrum s výrazným vlivem generačně-rekombinačních procesů. Další výrazná vlastnost degenerovaných detektorů a detektorů nižší kvality je nárůst spektrální hustoty šumu typu 1/f se vzrůstajícím napájecím napětí se směrnicí výrazně vyšší než 2. Strukturální a chemické analýzy poukázaly, že teplotní generace detektorů způsobuje difuzi kovu použitého při kontaktování a stopových prvků hlouběji do objemu krystalu. Část mé práce je věnována modifikaci povrchu svazkem argonových iontů a jejímu vlivu na chemické složení a morfologii povrchu.
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Modelling and Optimization of Complex Fiber Diffractive Structures
Helán, Radek ; Lazar,, Josef (referee) ; Tománek, Pavel (referee) ; Urban, František (advisor)
The thesis discusses the fiber Bragg gratings simulations, analysis and design. In the present time, there are several methods to simulate fiber gratings response based on the stated parameters that define their dimensions and material features. However, this work deals with a different issue, that is the synthesis of the input parameters for demanded spectral responses. The main aim of the work is to achieve a synthesis method that would help to discover parameters describing advanced grating structure, based on the required spectral reflectivity. The basic demand for the parameter synthesis is an achievement of the real values in terms of the consequent production of the suggested structure. The described synthesis method considers advanced fiber grating structure as a structure of several uniform grating sections. The input parameters are estimated in steps, using the well-known direct methods in order to obtain grating responses and feedback to establish the parameters changes. The principle methods involve establishment of initial input parameter values and necessary subsequent algorithm leading to optimize the required spectral response. The initial values are calculated by a simplified model based on the coupled theory equations that are handled for the periodic disturbances in cylindrical waveguide. The following optimization uses the multiple thin film stack and transfer matrix methods. The properties of grating structure spectral reflectivity are step by step calculated while using these direct methods. Input parameters are established in the next several steps. Establishment of input parameters is done subsequently, based on the demanded and calculated output spectral reflectivity properties. Optimizing process is limited by possibilities of the grating manufacture technology. It is possible to assemble arbitrary fiber grating structure taking in term the demanded spectral response. Nevertheless, the calculated input parameters are real for the following manufacture. This method could be used to design optical band stop filter, high-pass and low-pass filters or filters for special applications.
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Spatial Division of Optical Beams
Hampl, Martin ; Lazar, Josef (referee) ; Vlček, Čestmír (referee) ; Wilfert, Otakar (advisor)
The dissertation thesis deals with a free space optical links, especially is focused directly to the transmission optical beams. The influence of environmental conditions like atmospheric turbulence, buildings movement and vibration is described. As a possibility to reduce these undesirable influences, the generation of the transmission beam by the help of the two-mode optical fiber is proposed. A possibility to drive the shape of the resulting transmission beam by means of the change of power ration of individual modes in fiber is described. The coherence features of the proposed beam are analyzed considering the possibility to reduce the influence of atmospheric turbulence. In the thesis there are introduced beam quality parameters that enable to evaluate the quality of the optical beam for the free space optical link. These parameters give the relationship between the shape of the optical beam and the power balance of the optical link and the statistical evaluation of the optical link.
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Low level measurement
Roubal, Zdeněk ; Lazar, Josef (referee) ; Sládek, Petr (referee) ; Steinbauer, Miloslav (advisor)
The doctoral thesis presents methods for low-level measurements, which are exemplified by the general measurement of air ion concentration. Within the first section, the author progressively identifies certain critical effects influencing the concentration of air ions, and these effects are to be considered in evaluating the uncertainty of the measured concentrations. Thus, the thesis aims to provide a systematic description of the procedures and requirements related to low-level measurements; the proposed description then involves a discussion of the process reliability requirements, namely the factors of repeatability, elimination of errors, and definition and describability of measurement uncertainties. Such methodology facilitates, among other activities, the long-term monitoring of air ion concentration in extreme environments, including caves or spaces characterized by high temperatures and humidity. The use of common methods for determining the air ion mobility spectrum is accompanied by a number of disturbing effects, such as spurious fluctuation in the concentration of air ions in time. The experimental section of the thesis describes the testing of different configurations of the measuring device (a collecting electrode and a polarization capacitor); at this stage, we seek the most advantageous distribution of the electrostatic field in the sensor – a Gerdien tube. The systematic suppression of the influence of spurious fluctuations in air ion concentration during the measurement of saturation characteristics is carried out with the least-squares numerical method (as a supporting element), a hybrid technique to describe and evaluate the experimental model. In the second part of the thesis, the methodology of low-level measurement is complemented with procedures for the measurement of freezing potential. This latter domain, as an overall experimental approach applicable during phase changes of the sample, has hitherto not been sufficiently characterized from the metrological perspective, in an up-to-date manner; several subregions of the said domain remain somewhat obscured and deserve significantly more attention. In the given context, this thesis systematically considers a problem where comparable measurement results produced by multiple authors are not available. The measuring methodology proposed herein was utilized in applied research to facilitate the fabrication of an apparatus for the experimental measurement of freezing potential in pre-defined samples; the obtained results then lead us to conclusions with respect to the evaluation of effects that cause differences in the measured data, and the results can be further compared with those obtained by other researchers investigating the given segment of the problem.
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Polarization Imperfections of Light in Interferometry
Kučera, Petr ; Lazar, Josef (referee) ; Mohr, Fridemann (referee) ; Wilfert, Otakar (advisor)
Disertační práce pojednává o polarizačních nedokonalostech optických komponentů, které jsou využívány ke kontrole a k transformaci polarizačního stavu světla. Získané teoretické výsledky jsou pak využity ve vybraných aplikacích, jež ke své činnosti využívají právě polarizace světla. Konkrétně se jedná o zařízení měřící vibrace oscilujících objektů, dále o interferenční měření dvojlomu v transparentních materiálech a konečně, o vybraná témata z optické kvantové komunikace.
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Synchronized sources of clock signals
Florián, Antonín ; Lazar,, Josef (referee) ; Drexler, Petr (advisor)
This thesis deals with design of synchronized sources of time signal. The first part of the thesis deals with the theory the crystal resonators, crystal oscillators and PLL. The second part of the thesis deals with the crystal oscillator with 10MHz frequency. In the oscillator is use of SC cut crystal. The third part of the work deals with method of synchronization. To synchronization is use of PLL.
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Local optical and electrical characteristics of optoelectronic devices
Škarvada, Pavel ; Hrabovský, Miroslav (referee) ; Lazar, Josef (referee) ; Tománek, Pavel (advisor)
Solar energy conversion, miniaturization of semiconductor devices and associated lifetime, reliability and efficiency of devices are the basic premise of this work. This work is focused on the study of optoelectronic devices especially solar cells and its nondestructive diagnostic. Solar cells are advantageous for study mainly because the pn junction is located near the surface and contains a lot of inhomogeneities. It has been difficult until recently to investigate their local physical (electrical and optical) parameters due to the size of inhomogeneities. Behavior of inhomogeneities can be well understood with knowledge of its local properties. Establishment of measurement workplace, that satisfies requirements for measurement of local emission and optically induced current measurement, allows us detection and localization of inhomogeneities with spatial resolution more or less 100 nm. The core of thesis is characterization of imperfection using nondestructive techniques in the macroscopic region but primarily in microscopic region using scanning probe microscopy. Integral parts of the work are characterization techniques for photoelectrical devices, microscopic techniques and data processing. Scanning near-field optical microscope is used for the purpose of microscopic characterization such as topography, local optical, photoelectrical and electrooptical properties of structures in high spatial resolution. Locally induced current technique, current voltage characteristics, emission from reversed bias pn junction measurement including its thermal dependence are used for samples investigation in macroscopical region. It is possible to localize defects and structure inhomogeneity using mentioned techniques. Localised defects are consequently analyzed for composition and measured using electron microscopy. Specific outputs of work are classification of photoelectric devices defects and specification of nondestructive characterization techniques used for defect detection. Experimental characterization techniques are described together with defects measurement procedures. The key output is the catalog of serious defects which was detected. Particular defects of samples are shown including describe of its properties and physical meaning.
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Degradation of GaAs Solar Cells
Papež, Nikola ; Pinčík,, Emil (referee) ; Lazar, Josef (referee) ; Sobola, Dinara (advisor)
Solární články na bázi arsenidu gallia patří mezi nejvýkonější typ dostupných solárních článků vůbec. Jejich výhodou je výborná odolnost vůči tepelnému a ionizujícímu záření, a proto se využívají zejména v náročných podmínkách. Tato disertační práce popisuje stav GaAs fotovoltaických článku vystavených vůči tepelnému namáhání, vysokému ochlazování, gama záření a ozáření širokospektrálním laserem. Vzorky byly zkoumány před, po a i během těchto procesů pomocí několika analytických a charakterizačních metod. Měření bylo zaměřeno na charakterizaci povrchu, optických a elektrických vlastností. Byly objeveny limity a nové chování tohoto typu článků, které jsou ovlivněny i tenkými ochrannými a antireflexními vrstvami.
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