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Characterization of obesogenic diet effect on adipose depots in mice C57BL/6J
Matějková, Mirka ; Bardová, Kristina (advisor) ; Lacinová, Zdeňka (referee)
Obesity is closely related to insulin resistance development, ectopic fat accumulation in the liver and skeletal muscles, liver steatosis and type 2 diabetes mellitus. Adipose tissue of obese subjects produces increased quantity of adipokines, which are involved in development of insulin resistance, and in the same time macrophages causing low-grade inflammation are infiltrating into adipose tissue. Long-chain polyunsaturated fatty acids of the n-3 series have beneficial effects on adipose tissue inflammation and lipid and carbohydrate metabolic pathways in organism. There is lot of research ongoing on laboratory animals on negative consequences of obesity and possible improvement. Obesity is induced by diet with high fat content and the consequences are evaluated. I have analysed the influence of different high fat diets and a diet with long-chain polyunsaturated fatty acids of the n-3 series on homogeneity of adipose depots in the cell size and the level of inflammation. I observed gradual development of obesity due to administration of high fat diet to laboratory animals. Methods: Intraperitoneal glucose tolerance test (IPGTT), oral glucose tolerance test (OGTT), assessment of lipid metabolites in blood, assessment of triacylglycerols in the liver and in the adipose tissue, assessment of DNA in...
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