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The optimization and validation of HPLC method for determination of triamcinolone acetonide in topical pharmaceutical product
Krivda, Anton ; Matysová, Ludmila (advisor) ; Nováková, Lucie (referee)
Author: Krivda A. Title: The optimalisation and validation of HPLC method for determination of triamcinolone acetonide in topical pharmaceutical product Language: Czech Keywords: HPLC Triamcinolone acetonide Triamcinolone Methylparaben Propylparaben Validation A novel reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of active component triamcinolone acetonide, its degradation product triamcinolone and two preservatives presented in the cream - methylparaben and propylparaben, using estradiole as an internal standard. The chromatographic separation was performed on a Supelco Discovery HSF5 column. The mobile phase for separation of all compounds consists of a mixture of acetonitrile and water (45:55 v/v). The analysis time was less than 9 minutes, at a flow rate of 0,6 ml/min and detection at 240 nm. The method was found to be appicable for routine analysis (stability tests, homogeneity) in the pharmaceutical product topical cream Triamcinolone cream 0,1%.
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The optimization and validation of HPLC method for determination of triamcinolone acetonide in topical pharmaceutical product
Krivda, Anton ; Matysová, Ludmila (advisor) ; Nováková, Lucie (referee)
Author: Krivda A. Title: The optimalisation and validation of HPLC method for determination of triamcinolone acetonide in topical pharmaceutical product Language: Czech Keywords: HPLC Triamcinolone acetonide Triamcinolone Methylparaben Propylparaben Validation A novel reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of active component triamcinolone acetonide, its degradation product triamcinolone and two preservatives presented in the cream - methylparaben and propylparaben, using estradiole as an internal standard. The chromatographic separation was performed on a Supelco Discovery HSF5 column. The mobile phase for separation of all compounds consists of a mixture of acetonitrile and water (45:55 v/v). The analysis time was less than 9 minutes, at a flow rate of 0,6 ml/min and detection at 240 nm. The method was found to be appicable for routine analysis (stability tests, homogeneity) in the pharmaceutical product topical cream Triamcinolone cream 0,1%.
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The Usage of HPLC in Pharmaceutical Analysis
Krivda, Anton ; Matysová, Ludmila (advisor) ; Nováková, Lucie (referee)
Title: The usage of HPLC in pharmaceutical analysis Name: Anton Krivda This labour deals with determination and validation of method suitable for qualitative and quantitative analysis of dimetindeni maleas in Fenistil® gel trough the use of high performance liquid chromatography with UV detection. There were two differential analytical columns tested (C18 and ZICTM column). Acceptable outcomes were got with these chromatographic conditions: wavelength λ = 258 nm. analytical column: SeQuant ZICTM Column(50 x 2.1 mm; 5 μm) compound of mobile phase: acetonitrile : aqeous solution of acetic acid (25 mM) and ammonium acetate (2,5 mM), (87,5:12,5) flow rate fm = 0,3 ml/min internal standard: diltiazem hydrochloride Recovery, effectivity of column, assymetry and resolution of chromatographic peaks were tested in validation tehse method. Each of them reposed in licenced range.
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