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Comparison of the immunophenotypes of normal and pathological plasma cells - determination of the rate of risk of progression in patients with MGUS
Kozelka, Jiří ; Jílek, Petr (advisor) ; Jankovičová, Karolína (referee)
- 7 - Abstract Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Jiří Kozelka Supervisor: PharmDr. Petr Jílek, CSc. Consultant: Mgr. Vladimíra Vroblová, Ph.D. Title of diploma thesis: Comparison of the immunophenotypes of normal and pathological plasma cells - determination of the rate of risk of progression in patients with MGUS Four-color flow cytometry is a necessary part of plasma cell imunophenotypisation today. It allows to define plasma cell count with a high accuracy and to distinguish normal and pathologic cell on the base of surface and cytoplasmatic markers expression. An expression of the following surface markers, CD138, CD38, CD19, CD56, CD45 CD20 and CD27, was monitored in this dissertation. An expression of the cytoplasmatic light chain κ and λ followed which confirmed monoclonality of the plasma cells in patients with multiple myeloma or with monoclonal gammopathy of undetermined significance. In reference group of patients the expression of light chain κ and λ confirmed polyclonality. 47 samples of patients with diagnosed multiple myeloma or with monoclonal gammopathy of undetermined significance and 22 samples from patients with increased count of plasma cell, but without present hematological disease of plasma cells,...
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Logic of the Single European Army
Kozelka, Josef ; Sršeň, Radim (advisor) ; Dubský, Zbyněk (referee)
The current economic crisis has led the leaders of the European states to decrease their spending on defense. This paper argues that if this reduction in public spending is not offset by the increase in international cooperation than the military capability of the region is going to deteriorate in the near future. The argument that the defense budgets are decreasing is based on the analysis of the data available for the public defense expenditures. Further six case studies of EU states are presented showing that the potential decrease in capability is observable in a wide range of EU countries. The case for greater international cooperation is based on the economic theory of comparative advantage and benefits of competition in the defense market. Furthermore, academic literature supporting this hypothesis is also presented to the reader throughout the paper. The conclusion of this paper is that the current tendency of governmental politicians of EU countries to ignore the need for greater international cooperation in the defense sector is unsustainable and that if European Union wants to maintain its position as one of the world power blocks, systematic reform of the EU defense market will need to be introduced.
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Monte Carlo modelování radiolýzy bází DNA
Kozelka, Jakub ; Běgusová, Marie
An attempt to explain differences in measured yields of modified bases determined in free bases and in the double stranded DNA is made with help of theoretical calculations. A theoretical model is applied to predict yields of reactions of the most important radiolytic product, OH• radical, with individual nucleobases, nucleosides and short DNA oligomers. The relation between spatial limitations for radical approach to particular reaction sites within DNA and the yields of damaged bases is discussed.
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