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Abundance of NADP-dependent enzymes in plants
Kovaľová, Terézia ; Hýsková, Veronika (advisor) ; Ryšlavá, Helena (referee)
NADPH is an important molecule in plant metabolism. It is an indispensable substrate for many processes; participates in redox balance of the cell and it is a part of antioxidant defense system in plants. In this work, activities of selected NADP-dependent enzymes producing NADPH, NADP-malic enzyme (oxalacetate decarboxylating) (NADP-ME, EC 1.1.1.40), NADP-isocitrate dehydrogenase (decarboxylating) (NADP-ICDH, EC 1.1.1.42), glukosa-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) and shikimate dehydrogenase (SDH, EC 1.1.1.25) in various plants and their parts (leaves, fruits, seeds) were established and compared. The aim of this work was to select suitable model plants for testing the effect of abiotic stress on NADP-dependent enzymes and also to select the best source for purification of these enzymes. Cucumber (Cucumis sativa L.), pea (Pisum sativum L.), or water-lily (Nymphaea) were chosen as suitable water-receptive model plants and Capsella bursa-pastoris as xerophilic plant from 19 analyzed plants. From group of 39 plants the most suitable for purification of NADP-ME were roots of Chinese radish (Raphanus sativus var. Major), which were simultaneously a rich source of other analyzed NADP-enzymes. The best source of NADP-ICDH was poppy (Papaver somniferum), or quinoa Chenopodium quinoa. For...
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The influence of stress on NADP-dependent enzymes in higher plants.
Kovaľová, Terézia ; Hýsková, Veronika (advisor) ; Dračínská, Helena (referee)
Biotic stress in the form of viral infection, as well as abiotic salt stress, cause leaves injuries, stomata closure and decreased rate of photosynthesis. These factors lead to the limitation of plant growth and to reduced amount of coenzyme NADPH. However NADPH is an important coenzyme for many metabolic pathways such as synthesis of fatty acids, amino acids and secondary metabolites involved in stress responses. NADPH is also a coenzyme for key enzymes of antioxidant system and for many regulatory enzymes. NADP-dependent enzymes are alternative source of NADPH in plants under stress conditions. In this work, activities of four NADP-dependent enzymes: Glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49), NADP-isocitrate dehydrogenase (NADP-ICDH, EC 1.1.1.42), NADP-malic enzyme (decarboxylating) (NADP-ME, EC 1.1.1.40) and Shikimate dehydrogenase (SDH, EC 1.1.1.25) were studied. Activities of all these enzymes but SDH increased in leaves of tobacco plants (Nicotiana tabacum L.) infected by PVYNTN , The most sensitive enzymes to viral infection were NADP-ICDH and NADP-ME, whose activity was increased in comparison with control plants 3-fold and 2,4-fold, respectively. Changes in activity of studied enzymes were also determined in plants exposed to viral infection in combination with heat-shock...
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The influence of stress on NADP-dependent enzymes in higher plants.
Kovaľová, Terézia ; Hýsková, Veronika (advisor) ; Dračínská, Helena (referee)
Biotic stress in the form of viral infection, as well as abiotic salt stress, cause leaves injuries, stomata closure and decreased rate of photosynthesis. These factors lead to the limitation of plant growth and to reduced amount of coenzyme NADPH. However NADPH is an important coenzyme for many metabolic pathways such as synthesis of fatty acids, amino acids and secondary metabolites involved in stress responses. NADPH is also a coenzyme for key enzymes of antioxidant system and for many regulatory enzymes. NADP-dependent enzymes are alternative source of NADPH in plants under stress conditions. In this work, activities of four NADP-dependent enzymes: Glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49), NADP-isocitrate dehydrogenase (NADP-ICDH, EC 1.1.1.42), NADP-malic enzyme (decarboxylating) (NADP-ME, EC 1.1.1.40) and Shikimate dehydrogenase (SDH, EC 1.1.1.25) were studied. Activities of all these enzymes but SDH increased in leaves of tobacco plants (Nicotiana tabacum L.) infected by PVYNTN , The most sensitive enzymes to viral infection were NADP-ICDH and NADP-ME, whose activity was increased in comparison with control plants 3-fold and 2,4-fold, respectively. Changes in activity of studied enzymes were also determined in plants exposed to viral infection in combination with heat-shock...
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Abundance of NADP-dependent enzymes in plants
Kovaľová, Terézia ; Hýsková, Veronika (advisor) ; Ryšlavá, Helena (referee)
NADPH is an important molecule in plant metabolism. It is an indispensable substrate for many processes; participates in redox balance of the cell and it is a part of antioxidant defense system in plants. In this work, activities of selected NADP-dependent enzymes producing NADPH, NADP-malic enzyme (oxalacetate decarboxylating) (NADP-ME, EC 1.1.1.40), NADP-isocitrate dehydrogenase (decarboxylating) (NADP-ICDH, EC 1.1.1.42), glukosa-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) and shikimate dehydrogenase (SDH, EC 1.1.1.25) in various plants and their parts (leaves, fruits, seeds) were established and compared. The aim of this work was to select suitable model plants for testing the effect of abiotic stress on NADP-dependent enzymes and also to select the best source for purification of these enzymes. Cucumber (Cucumis sativa L.), pea (Pisum sativum L.), or water-lily (Nymphaea) were chosen as suitable water-receptive model plants and Capsella bursa-pastoris as xerophilic plant from 19 analyzed plants. From group of 39 plants the most suitable for purification of NADP-ME were roots of Chinese radish (Raphanus sativus var. Major), which were simultaneously a rich source of other analyzed NADP-enzymes. The best source of NADP-ICDH was poppy (Papaver somniferum), or quinoa Chenopodium quinoa. For...
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