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Optimization of drug-loaded nanoparticles preparation
Kolářová, Adéla ; Holas, Ondřej (advisor) ; Paraskevopoulos, Georgios (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Consultant: PharmDr. Ondřej Holas, PhD. Student: Adéla Kolářová Title of Thesis: Optimization of drug-loaded nanoparticles preparation At the beginning of the work there are described various types of nanoparticles, the polymer particles are described in more detail, which is also dealt with in the experimental part. Furthermore, possible methods of preparation of polymer nanoparticles and their use in medicine are listed. Two methods of nanoparticles preparation from materials based on of poly (lactic-co-glycolic acid) were used. First method was emulsion-sonication, where the particles were formed using high frequency ultra-sound probe. The second method was spontaneous emulsification, where two solvents with different hydrophilicity were used. Different properties of resulting nanoparticles were assayed - size, polydisperzity, encapsulation efficacy, drug loading and recovery yield. Rhodamine B, a fluorescent dye, was used as a model substance for encapsulation experiments Nanoparticles were prepared from linear PLGA and PLGA branched by polyacrylic acid. Nanoparticles were stabilized by three different surfactants - poloxamer Pluronic® F127, polysorbate Tween® 20 and poly(vinyl)alcohol. Each...
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