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General Runoff Water Balance Model of a River Basin
Černý, Vojtěch ; Knoppová, Kateřina (referee) ; Marton, Daniel (advisor)
Modelling of the rainfall-runoff process is one of the basic scientific skills in hydrology. Rainfall-runoff modelling can help to improve water management, handling of the reservoir's storage volume, or also to facilitate adaptation to current climatic conditions. The aim of the diploma thesis is to create a functional rainfall-runoff model on the basis of water balance equations based on the lumped water balance principle of the hydrological model. Several modifications of the general rainfall-runoff model are approached in the diploma thesis. Four types of the daily evapotranspiration determination are used in the calculations. The rainfall-runoff model is compiled from temperature data and precipitation totals in a daily step. The practical application is carried out on a sub-basin of the river Dyje, which is located above Vranov water reservoir. The main output is a series of daily flow rates that were obtained from calibrated rainfall-runoff models. The best rainfall-runoff model takes into account the water from snow cover melting, the value of the Nash Sutcliffe calibration criterion of this model is 0.608. Finally, the hydrological simulation for the period 2021-2060 is performed in the diploma thesis.
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Water Management Analysis of Reservoir Storage Capacity Under Climate Change
Smolík, Lukáš ; Knoppová, Kateřina (referee) ; Marton, Daniel (advisor)
The thesis is processed from several partial steps. The first step is to calibrate and validate the hydrological model using real meteorological and hydrological data using the balance hydrological model in Runoff Prophet. Furthermore, using the corresponding statistical downscaling in the LARS WG program. Create climatological data sets considering climate change. Hydrological transformation of climatological data by hydrological model to compile sets of hydrological series for Vlára river basin. Recalculate the hydrological data to the body of the dam for the tributary of the Vlára River, including water transfers from the basins of the Sviborka and Smolinka streams. The last step of the work is to analyze the storage volume and its respective improved water outflow from the tank and to assess the impact of climate change on the storage volume of the tank.
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Influence of albendazole on cell proliferation in vitro
Knoppová, Kateřina ; Szotáková, Barbora (advisor) ; Jirkovská, Anna (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Kateřina Knoppová Supervisor: Doc. Ing. Barbora Szotáková, Ph.D. Supervisor specialist: RNDr. Věra Králová, Ph.D. Title of diploma thesis: Influence of albendazole on cell proliferation in vitro Albendazole (ABZ) is a benzimidazole anthelmintic widely used for the treatment of parasitic diseases in man and animals. The mechanism of action is based on inhibition of microtubule polymerization by binding of ABZ to the β-subunit of tubulin in parasites. As a result, it subsequently leads to the disruption of vital functions of the parasite and to its death. After elucidation of this mechanism, ABZ was tested as a potential anticancer agent. It has been shown that it is able to inhibit proliferation of some cancer cell lines. In this study, we examined the effect of ABZ on cell proliferation in vitro. For the purpose of our experiment we used cell lines derived from colorectal carcinoma - SW 480 and SW 620 together with a cell line NCM 460 isolated from normal colonic mucosa. The cells were treated with ABZ in the concentration range of 0.01 - 10 µM for 24, 48 and 72 hours. Cell viability was determined using WST-1 assay and Neutral Red Uptake Test (NRU). ABZ inhibited cell proliferation...
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General Runoff Water Balance Model of a River Basin
Černý, Vojtěch ; Knoppová, Kateřina (referee) ; Marton, Daniel (advisor)
Modelling of the rainfall-runoff process is one of the basic scientific skills in hydrology. Rainfall-runoff modelling can help to improve water management, handling of the reservoir's storage volume, or also to facilitate adaptation to current climatic conditions. The aim of the diploma thesis is to create a functional rainfall-runoff model on the basis of water balance equations based on the lumped water balance principle of the hydrological model. Several modifications of the general rainfall-runoff model are approached in the diploma thesis. Four types of the daily evapotranspiration determination are used in the calculations. The rainfall-runoff model is compiled from temperature data and precipitation totals in a daily step. The practical application is carried out on a sub-basin of the river Dyje, which is located above Vranov water reservoir. The main output is a series of daily flow rates that were obtained from calibrated rainfall-runoff models. The best rainfall-runoff model takes into account the water from snow cover melting, the value of the Nash Sutcliffe calibration criterion of this model is 0.608. Finally, the hydrological simulation for the period 2021-2060 is performed in the diploma thesis.
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Water Management Analysis of Reservoir Storage Capacity Under Climate Change
Smolík, Lukáš ; Knoppová, Kateřina (referee) ; Marton, Daniel (advisor)
The thesis is processed from several partial steps. The first step is to calibrate and validate the hydrological model using real meteorological and hydrological data using the balance hydrological model in Runoff Prophet. Furthermore, using the corresponding statistical downscaling in the LARS WG program. Create climatological data sets considering climate change. Hydrological transformation of climatological data by hydrological model to compile sets of hydrological series for Vlára river basin. Recalculate the hydrological data to the body of the dam for the tributary of the Vlára River, including water transfers from the basins of the Sviborka and Smolinka streams. The last step of the work is to analyze the storage volume and its respective improved water outflow from the tank and to assess the impact of climate change on the storage volume of the tank.
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Influence of albendazole on cell proliferation in vitro
Knoppová, Kateřina ; Szotáková, Barbora (advisor) ; Jirkovská, Anna (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Kateřina Knoppová Supervisor: Doc. Ing. Barbora Szotáková, Ph.D. Supervisor specialist: RNDr. Věra Králová, Ph.D. Title of diploma thesis: Influence of albendazole on cell proliferation in vitro Albendazole (ABZ) is a benzimidazole anthelmintic widely used for the treatment of parasitic diseases in man and animals. The mechanism of action is based on inhibition of microtubule polymerization by binding of ABZ to the β-subunit of tubulin in parasites. As a result, it subsequently leads to the disruption of vital functions of the parasite and to its death. After elucidation of this mechanism, ABZ was tested as a potential anticancer agent. It has been shown that it is able to inhibit proliferation of some cancer cell lines. In this study, we examined the effect of ABZ on cell proliferation in vitro. For the purpose of our experiment we used cell lines derived from colorectal carcinoma - SW 480 and SW 620 together with a cell line NCM 460 isolated from normal colonic mucosa. The cells were treated with ABZ in the concentration range of 0.01 - 10 µM for 24, 48 and 72 hours. Cell viability was determined using WST-1 assay and Neutral Red Uptake Test (NRU). ABZ inhibited cell proliferation...
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Laboratory manual and standard operating techniques in the hematology section of OKBH, Panoch's hospital s.r.o., Turnov.
Knoppová, Kateřina ; Jílek, Petr (advisor) ; Křivčíková, Lucie (referee)
This bachelor's thesis is focused mainly on the verification of methods and on the standardized operating procedures, which are required in the conditions of accreditation. The main purpose of this thesis was a contribution to the elaboration of the laboratory manual and the preparation of the directed documentation, mainly the elaboration of the standardized operating procedures and the verification of methods for the hematological department. The thesis is divided into the theoretical and the practical part. The theoretical part presents the laboratory and the accreditation process which the Department of the Clinical Biochemistry and Hematology is preparing for. Furthermore, it describes various analyzers from the hematological section of the laboratory and the most frequently executed methods. We created the standardized operating procedures for these methods, verified them and created the verification protocols. Due to the huge amount of the documents we present here two standardized operating procedures and two verification protocols.
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