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Determination of selenium and manganese in cerebrospinal fluid by atomic absorption spectrometry
Jiráková, Lucie ; Kotaška, Karel (advisor) ; Klapková, Eva (referee)
The aim of this study was to confirm the use of electrothermal atomic absorption spectrometry for the determination of selenium and manganese in the cerebrospinal fluid and to investigate concentrations of selenium and manganese in selected group of patients. For selenium was determined the detection limit 2,9 μg/l, for manganese 0,26 μg/l. 73 patients were examined (31 women, 42 men) whose average age was 14,1 years. The patients were divided into two groups according to age (56 children, 17 adults) and into two subgroups according to diagnoses (oncological, neurological). The control group consists of 18 subjects (5 women, 13 men, average age 21,7 years) with non-oncological and non-neurological diagnoses. We found significantly increased selenium concentrations in cerebrospinal fluid in the control group, compared with the group with neurological diagnoses (median = 14,4 μg/l vs. 12,4 μg/l, p < 0.05). Elevated levels of manganese in cerebrospinal fluid was observed in a group of children with oncological diseases compared with control group (1,2 μg/l vs. 0,5 μg/l, p < 0.05). Determination of selenium and manganese in the cerebrospinal fluid may have diagnostic importance in selected groups of patients. Powered by TCPDF (www.tcpdf.org)
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Use of biochemical markers (Lipoprotein phospholipase A2 and hyaluronic acid) for laboratory diagnosis of metabolic and degenerative diseases of the musculoskeletal system
Kotaška, Jan ; Trč, Tomáš (advisor) ; Klapková, Eva (referee) ; Karpaš, Karel (referee)
Use of biochemical markers (Lipoprotein phospholipase A2 and hyaluronic acid) for laboratory diagnosis of metabolic and degenerative diseases of the musculoskeletal system Abstract Musculoskeketal disorders currently belong to the most common diseases. The presented work describes the use of biochemical markers (Lipoprotein phospholipase A2 and hyaluronic acid) for laboratory diagnosis of metabolic and degenerative diseases of the musculoskeletal system. Concentrations of LP-PLA2 were significantly elevated in the patients with bone resorption compared to the control group of healthy individuals. Serum levels of Lp-PLA2 also negatively correlated with decreased levels of serum osteocalcin in patients. HA concentrations in synovial fluid did not differ from published reference values in synovial fluid. Patients who underwent arthroscopy had significantly elevated synovial HA concentration than patients who underwent total knee endoprosthesis. HA positively correlates with osmotic pressure determined by examination of osmolality in synovial fluid. Lipoprotein phospholipase A2 concentrations are elevated in patients with bone density impairment. LpPLA2 concentrations correlate with the severity of bone density impairment expressed by the T score. Hyaluronic acid concentrations in patients with knee...
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Biochemistry and pathobiochemistry of phylloquinone and menaquinones
Dunovská, Kateřina ; Klapková, Eva (advisor) ; Uřinovská, Romana (referee) ; Skoumalová, Alice (referee)
Vitamin K belongs to the family of fat-soluble vitamins, which is not determinated in clinical laboratories. It is a cofactor necessary for posttranslational γ-carboxylation of glutamyl residues in selected proteins such as the osteocalcin, and procoagulation factors II, VII, IX, X. Vitamin K deficient individuals appear to have more undercarboxylated proteins, which are functionally defective. Lack of this vitamin has been associated with risk of developing osteoporosis and cardiovascular diseases. The aim of this work was to develop and validate the HPLC method and the LC-MS/MS method for determination of three vitamin K's forms - vitamin K1, MK-4 and MK-7 in serum. After successful validation of both methods, patient samples and healthy population samples were measured. There were measured 350 patient samples by HPLC method. These samples were divided into two groups - patients with diagnostic of osteoporosis and patients without osteoporosis. We measured 946 samples by LC-MS/MS method. Samples were divided into groups: patients with osteoporosis, patients without osteoporosis, healthy population, patients with osteopenia and patients with cystic fibrosis. The reference range of vitamin K in healthy population was obtained by LC-MS/MS method. The next aim was to compare the effectiveness of...
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Immunochemical determination of active and inactive form of cathepsin B in patients with bladder cancer
Urban, Tomáš ; Bosáková, Zuzana (advisor) ; Klapková, Eva (referee)
This thesis is focused on immunochemical determination of concentration of active and inactive form of cathepsin B in patients with bladder cancer in order to compare diagnostic efficiency of methods for their possible use for routine diagnosis. Cathepsin B and procathepsin B were measured in serum and urine in 82 patients with bladder cancer (47 men and 35 women), with the average age of 66.5 year. The control group contain of 72 healthy subjects (31 men and 41 women), with the average age of 58.5 year. The concentration of cathepsin B and procathepsin B in the urine were corrected to creatinine, which was determined by the enzymatic creatinase method. The concentrations of cathepsin B in urine were singnificantly elevated in patients than in control group (median = 3.5 µg/l vs. 0.9 µg/l, P = 0.01), similarly the results of the cathepsin B/creatinine ratio were elevated (median = 0.4 µg/mmol vs. 0.1 µg/mmol, P = 0.01). There were no significant difference in concentration in serum between patients and control group (median = 4.8 µg/l vs. 4.2 µg/l, P = 0.8). The concentration values of procathepsin B were significantly higher in patients compare to control group both in urine (median = 3.9 µg/l vs. 1.4 µg/l, P < 0.0001), in serum (median = 73.3 µg/l vs. 58.7 µg/l, P = 0.0005) and similarly in...
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Determination of vancomycin and its crystalline degradation products by HPLC
Neščáková, Monika ; Klapková, Eva (advisor) ; Kotaška, Karel (referee)
The aim of this bachelor thesis was to introduce and validate the method for the determination of vancomycin and its crystalline degradation products by high performance liquid chromatography with detection by diode-array detector. This method is able to reliably detect and distinguish the crystalline degradation products of vancomycin, which lack the necessary therapeutic effect. The long-term using of vancomycin or renal insufficiency lead to their accumulation in the patient's body. Another target of this bachelor thesis was to monitor the release profile of vancomycin concentration and its crystalline degradation products from local carrier of antibiotics that are used in some complicated orthopedic surgeries such as prevention of inflammatory infections caused by Staphylococcus aureus. We compared in vitro release of vancomycin from two bone grafts, two different bone cements and from one synthetic bone graft. The aim was to determine if the examine material releases the quantity of vancomycin, which ensure the long-term local concentrations of vancomycin higher than is the MIC of vancomycin-resistant Staphylococcus aureus (VRSA). Powered by TCPDF (www.tcpdf.org)
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Determination of selenium and manganese in cerebrospinal fluid by atomic absorption spectrometry
Jiráková, Lucie ; Kotaška, Karel (advisor) ; Klapková, Eva (referee)
The aim of this study was to confirm the use of electrothermal atomic absorption spectrometry for the determination of selenium and manganese in the cerebrospinal fluid and to investigate concentrations of selenium and manganese in selected group of patients. For selenium was determined the detection limit 2,9 μg/l, for manganese 0,26 μg/l. 73 patients were examined (31 women, 42 men) whose average age was 14,1 years. The patients were divided into two groups according to age (56 children, 17 adults) and into two subgroups according to diagnoses (oncological, neurological). The control group consists of 18 subjects (5 women, 13 men, average age 21,7 years) with non-oncological and non-neurological diagnoses. We found significantly increased selenium concentrations in cerebrospinal fluid in the control group, compared with the group with neurological diagnoses (median = 14,4 μg/l vs. 12,4 μg/l, p < 0.05). Elevated levels of manganese in cerebrospinal fluid was observed in a group of children with oncological diseases compared with control group (1,2 μg/l vs. 0,5 μg/l, p < 0.05). Determination of selenium and manganese in the cerebrospinal fluid may have diagnostic importance in selected groups of patients. Powered by TCPDF (www.tcpdf.org)
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