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Na/K-ATPase in spleen lymphocytes and hippocampus of rat; effect of morphine, stimulation by mitogen and effect of stress induced by deprivation from sleep
Kaufman, Jonáš ; Svoboda, Petr (advisor) ; Hejnová, Lucie (referee)
This work was oriented to studies of sodium and potassium dependent adenosinetriphosphatase, Na+ /K+ ATPase, which is selectively inhibited by cardioactive glycoside, ouabain. The alterations in level of this enzyme were followed in spleen lymphocytes and hippocampus prepared from rats. Detection of Na+ /K+ ATPase has been made by western blot analysis using primary antibodies oriented against α subunit of Na+ /K+ ATPase. Studies of lymphocytes were based on usage of both monoclonal and polyclonal antibodies. In studies of hippocampus monoclonal antibodies were used. The first aim of my work was to determine the alterations in the level of Na+ /K+ ATPase in spleen lymphocytes cultivated in tissue culture (i.e. under in vitro conditions) in the presence of morphine or strong mitogen, concanavalin A (ConA). The second aim of these theses was to determine the changes of Na+ /K+ ATPase α subunit in hippocampus of rats, which were under in vivo conditions exposed to stress lasting 3 days. The stress of experimental animals was induced by deprivation from sleep. The long-term incubation of spleen lymphocytes with 10 μM morphine for 48 hours did not cause a significant change of Na+ /K+ ATPase α subunit level. This result was obtain by analysis of post nuclear fraction (PNS), by use of monoclonal...
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The impact of intracytoplasmic sperm injection on succesful development and fertility in selected mammal species
Kaufman, Jonáš ; Petelák, Aleš (advisor) ; Petr, Jaroslav (referee)
5 Abstract This thesis aims to summarize the current knowledge about the development and reproductive capacities of individuals conceived by artificial conception, especially intracytoplasmic sperm injection (ICSI), and to make a comparison with naturally conceived ones. The focus is primarily on human assisted reproduction, even though scientific work with human gametes and embryos faces ethical difficulties. However, the main objective is to determine whether ICSI has a negative effect on offspring, partially using results from animal models. Furthermore, this thesis compares birth outcomes, incidence of birth defects, cognitive and reproductive abilities and both genetic and epigenetic integrity of individuals conceived naturally with those conceived via ICSI. As of yet, according to available information, ICSI appears to be a safe artificial method of reproduction. Poor results may often either be explained by the reduced fertility of parents, or their significance is negligible. Keywords: artificial fertilization, ART, ICSI, reproduction, offspring, mouse, livestock, human
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