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Comparison conductive detectors for capillary electrophoresis
Kříček, Zdeněk ; Křížek, Tomáš (advisor) ; Kubíčková, Anna (referee)
The aim of this bachelor thesis is comparison of two contactless conductivity detectors in capillary electrophoresis. Two methods were used for comparison: The determination of saccharides, namely glucose, fructose and sucrose, and then the determination of inorganic ions, such as chlorides, nitrates, and sulphates. The theoretical part deals with capillary electrophoresis in general and then the specific use of a contactless conductivity detector in capillary electrophoresis and its optimization. Parameters used for comparison of detectors such as limit of detection or relative standard deviation are described. Afterwards, different methods that can be used for the separation and determination of saccharides and inorganic ions are reviewed. The experimental part contains data that were measured in the optimization part of this study as well as comparison of the two detectors used, Admet and TraceDec. The comparison part starts with the method for the separation of saccharides using short-end injection mode in 40mmol∙l−1 sodium hydroxide with a separation voltage of −15 kV. The internal standard was 0,1mg∙ml−1 lactose. The calibration curve was measured in the concentration range from 0,01 to 0,5 mg∙ml−1 The linearity was measured with values of LOD, LLOQ and RSD determined as well. The values of...
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