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New possibilities of providing basic natural science education through experiments
Köhlerová, Veronika ; Beneš, Pavel (advisor) ; Liška, František (referee) ; Dvořáček, Jiří (referee)
TITLE: New possibilities of providing basic natural science education through experiments AUTHOR: Mgr. Veronika Köhlerová DEPARTMENT: Department of Chemistry and Chemistry Education SUPERVISOR: Prof. RNDr. Pavel Beneš, CSc. ABSTRACT: The thesis stems from the basic need to support and develop natural science education. Based on didactic, technical-economic and ergonomic requirements, a teaching tools kit has been created, which facilitates 100 natural science experiments. The kit includes a methodological manual; further methodological support is also provided on the webpage of the kit's manufacturer. To complement the kit, a concept of an optional teacher training course Natural Science Experiments for Preschools and Primary Schools has been created. The course will take place at the Pedagogical Faculty of Charles University in the school year 2013/2014. During the course of the thesis, didactic research methods were used to choose, process, test, organize, and optimise educational experiments. The pedagogical methods used included questionnaires and expert testimonials. The thesis provides a substantial response to the current educational needs in the field of natural science pre-literacy already at preschools and natural science literacy at primary schools. KEYWORDS: science experiment, natural science...
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Identification of Protein Binders Contained in the Artworks by gas Chromatography
Köhlerová, Veronika ; Beneš, Pavel (referee) ; Hrdličková Kučková, Štěpánka (advisor)
Painters often used a protein binding agents such as various types of animal glues, casein or eggs, either whole or their individual parts for preparation of many different types of temperas. The determination of the proteinaceous binder is essential in order to choose a suitable conservation method and for identification of the painting technique. This diploma thesis aims to define the most suitable conditions to determine the proteinaceous binders by gas chromatography with flame ionisation detector (GC-FID). Firstly, the optimal conditions needed for separation of individual amino acids, which are necessary to distinguish the protein binding agents, were set. Secondly, the empirically define conditions for hydrolysis of proteins, which breaks down proteins into the individual amino acids, were found.
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