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The effect of carbon nanostructures on human cell behavior and the role of fetal bovine serum in cell adhesion
Jannová, Martina
Graphene (G) and nanocrystalline diamond (NCD) are carbon allotropes and promising nanomaterials with an excellent combination of their properties, such as high mechanical strength, electrical and thermal conductivity, possibility of functionalization and very high surface area to volume ratio. For these reasons, G and NCD are employed next to electronics in biomedical applications, including implant coating, drug and gene delivery and biosensing. For a fundamental characterization of cell behavior on G and NCD, we studied osteoblast adhesion and proliferation on differently treated G and NCD. Generally, both G and NCD exhibited better properties for osteoblast cultivation than control tissue culture polystyrene. Better cell adhesion but lower cell proliferation were observed on NCD compared to G. The most surprising finding was that hydrophobic G with nanowrinkled topography enhanced cell proliferation extensively, in comparison to hydrophilic and flat G and both NCDs (hydrophobic and hydrophilic) with slightly higher roughness. Promoted cell proliferation enables faster cell colonization of G and NCD substrates, meaning faster new tissue formation which is beneficial in biomedical applications. Furthermore, it was shown that osteoblast adhesion was promoted in the initial absence of fetal bovine...
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The effect of carbon nanostructures on human cell behavior and the role of fetal bovine serum in cell adhesion
Jannová, Martina
Graphene (G) and nanocrystalline diamond (NCD) are carbon allotropes and promising nanomaterials with an excellent combination of their properties, such as high mechanical strength, electrical and thermal conductivity, possibility of functionalization and very high surface area to volume ratio. For these reasons, G and NCD are employed next to electronics in biomedical applications, including implant coating, drug and gene delivery and biosensing. For a fundamental characterization of cell behavior on G and NCD, we studied osteoblast adhesion and proliferation on differently treated G and NCD. Generally, both G and NCD exhibited better properties for osteoblast cultivation than control tissue culture polystyrene. Better cell adhesion but lower cell proliferation were observed on NCD compared to G. The most surprising finding was that hydrophobic G with nanowrinkled topography enhanced cell proliferation extensively, in comparison to hydrophilic and flat G and both NCDs (hydrophobic and hydrophilic) with slightly higher roughness. Promoted cell proliferation enables faster cell colonization of G and NCD substrates, meaning faster new tissue formation which is beneficial in biomedical applications. Furthermore, it was shown that osteoblast adhesion was promoted in the initial absence of fetal bovine...
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Bulvarization of Bussiness Weaklies
Jannová, Martina ; Řehořová, Irena (advisor) ; Poštulka, Filip (referee)
Today's audience tends to focus increasingly on popular media and on sensational and entertaining ways of media communication. Many authors describe present media culture as popular. There is a significant merge of information and entertainment known as infotainment: the entertaining news. Television practices model this trend, permeating widely into other media. Simultaneously, there is a slump in printed media caused by the quick entrance and fast spread of electronic media, especially the Internet and television. Both these circumstances are integrated in this thesis as well as in real world of periodical publishers. By making their titles more popular, the publishers of print media are trying to fight against sales decreases affecting the majority of reputable newspapers, weeklies and monthlies by making their titles more popular. The key sign of this popularization is a constant effort to increase both the semantic and the visual attractiveness of the periodicals. Consequently, it also causes higher uniformity of printed titles and a homogenization across this entire field: the printed media increasingly submit to its audience. The way of selecting and processing the information is almost identical, with the repetitive usage of well-tried and popular type of news.
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