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Study of properties of voltage membrane sensor ASAP1 expressed in HEK293 cell line
Jablonská, Dominika ; Čmiel, Vratislav (referee) ; Svoboda, Ondřej (advisor)
This thesis deals with the problematice of measuring membrane potential and monitoring the propagation of electrical activity of cells. For this purpose, fluorescence membrane voltage sensors have been developed to detect changes in the membrane potential by changing their fluorescence intensity. The practical part is focused on the study of the properties of the ASAP1 fluorescence probe, which was transfected into the HEK293 cell line, which are kidney cells from the human embryo. Cell membrane potential was changed using the patch-clamp technique.
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Robotic hand control by myopotentials
Jablonská, Dominika ; Janoušek, Oto (referee) ; Bubník, Karel (advisor)
The thesis deals with the measurement of myopotecials and their using in controlling the robotic arm. First, it is theoretically described electromyographic signal, his scanning and subsequent processing. There is also described an arm which it is used here. The next aim of this project is paying attention scanning signal on human hands using Biopac system and signal processing in MATLAB with Robotic Toolbox. Also was created a computer model in the user interface GUI.
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Study of properties of voltage membrane sensor ASAP1 expressed in HEK293 cell line
Jablonská, Dominika ; Čmiel, Vratislav (referee) ; Svoboda, Ondřej (advisor)
This thesis deals with the problematice of measuring membrane potential and monitoring the propagation of electrical activity of cells. For this purpose, fluorescence membrane voltage sensors have been developed to detect changes in the membrane potential by changing their fluorescence intensity. The practical part is focused on the study of the properties of the ASAP1 fluorescence probe, which was transfected into the HEK293 cell line, which are kidney cells from the human embryo. Cell membrane potential was changed using the patch-clamp technique.
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|
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Robotic hand control by myopotentials
Jablonská, Dominika ; Janoušek, Oto (referee) ; Bubník, Karel (advisor)
The thesis deals with the measurement of myopotecials and their using in controlling the robotic arm. First, it is theoretically described electromyographic signal, his scanning and subsequent processing. There is also described an arm which it is used here. The next aim of this project is paying attention scanning signal on human hands using Biopac system and signal processing in MATLAB with Robotic Toolbox. Also was created a computer model in the user interface GUI.
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