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Analysis of phenolics in different kinds of coffee
Jašková, Martina ; Halienová, Andrea (referee) ; Márová, Ivana (advisor)
The aim of this bachelor thesis was examining the quantity of biologically significant substances in selected types of roasted ground coffee, instant coffee and coffee extract. The particular kinds of coffee differed in technological processing, degree of roasting and composition. In the practical part total phenolics and flavonoids by using spectrophotometry methods. The total antioxidant activity in the individual coffee extracts was determined by ABTS method. Qualitative and quantitative analysis of some individual antioxidants and caffeine was performed using RP-HLPC with spectrophotometric detection. As a part of the work sensory analysis of all coffee samples was realized and consumer questionnaires were completed.
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Analysis of active substances in instant beverages for child´s nutrition
Jašková, Martina ; Kočí, Radka (referee) ; Márová, Ivana (advisor)
The aim of presented diploma thesis was to characterize baby beverages for early infant feeding. Theoretical part deals with baby foods and beverages including breast milk. Practical part is focused on analysis of active substances in 21 commercially available fruit and herbal instant granular baby beverages and in 3 packaged tea bags for infant and toddler age. To evaluation of antioxidant properties some group parameters were used - total polyphenols, total flavonoids, total antioxidant activity and anthocyanins. All of them were analysed by using spectrophotometrical methods. For identification and quantification of individual phenolic substances was used RP-HPLC/UV-VIS and HPLC/PDA/LC-MS, identification of individual mono- and disaccharides was performed by HPLC/RI. L-ascorbic acid supplemented to some children's drinks was analysed by HPLC-NH2/UV. Protein analysis was performed by Experion microfluidic electrophoresis system (BioRad) and protein concentration was verified by colorimetric method. In general, the highest content of phenolic compounds was shown in tea bags when compared with instant granular beverages that contain mostly added simple carbohydrates. Using contact microbial tests proved immediately after opening and also during manipulation with tea boxes was verified that analysed baby teas are in agreement with strict hygiene requirements for baby beverages.
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Establishment of the dependance of detection time in BacT/Alert SA bottles on a size of inoculum.
Jašková, Martina ; Buchta, Vladimír (advisor) ; Lesná, Jiřina (referee)
1 Summary This work deals with the sepsis problems and possibilities of its diagnostics with the help of a quantitative bacteriological investigation using hemocultural systems of BacT/Alert and Bactec. We focused on the yeasts genus Candida and tested the five most frequent species. The first part is dedicated to a theoretical introduction of the whole issue concerning the blood-course and the sepsis. It analyses the pathogeny of these infections and its generators, sepsis clasification and risk factors. It also mentions the diagnostic methods, both clinical and laboratory. Then the following part of the theoretical text is concerned with the hemoculture and automatic hemocultural systems BacT/Alert and Bactec. The practical part shows the used method description as well as the materials and tools. There is also a detailed work process description. The result part contains various graphs and tables of the dependance of the times to detection on the amount of the inoculum yeasts into hemocultural small bottles. The results show that the times to detection become shorter with the increasing amount of inoculum micro-organisms. Both systems deliver equivalent results. The growth curves designed by us could be used in a clinical practice for a quantitative determination of fungemia and a more sophisticated...
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Establishment of the dependance of detection time in BacT/Alert SA bottles on a size of inoculum.
Jašková, Martina ; Buchta, Vladimír (advisor) ; Lesná, Jiřina (referee)
1 Summary This work deals with the sepsis problems and possibilities of its diagnostics with the help of a quantitative bacteriological investigation using hemocultural systems of BacT/Alert and Bactec. We focused on the yeasts genus Candida and tested the five most frequent species. The first part is dedicated to a theoretical introduction of the whole issue concerning the blood-course and the sepsis. It analyses the pathogeny of these infections and its generators, sepsis clasification and risk factors. It also mentions the diagnostic methods, both clinical and laboratory. Then the following part of the theoretical text is concerned with the hemoculture and automatic hemocultural systems BacT/Alert and Bactec. The practical part shows the used method description as well as the materials and tools. There is also a detailed work process description. The result part contains various graphs and tables of the dependance of the times to detection on the amount of the inoculum yeasts into hemocultural small bottles. The results show that the times to detection become shorter with the increasing amount of inoculum micro-organisms. Both systems deliver equivalent results. The growth curves designed by us could be used in a clinical practice for a quantitative determination of fungemia and a more sophisticated...
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Analysis of active substances in instant beverages for child´s nutrition
Jašková, Martina ; Kočí, Radka (referee) ; Márová, Ivana (advisor)
The aim of presented diploma thesis was to characterize baby beverages for early infant feeding. Theoretical part deals with baby foods and beverages including breast milk. Practical part is focused on analysis of active substances in 21 commercially available fruit and herbal instant granular baby beverages and in 3 packaged tea bags for infant and toddler age. To evaluation of antioxidant properties some group parameters were used - total polyphenols, total flavonoids, total antioxidant activity and anthocyanins. All of them were analysed by using spectrophotometrical methods. For identification and quantification of individual phenolic substances was used RP-HPLC/UV-VIS and HPLC/PDA/LC-MS, identification of individual mono- and disaccharides was performed by HPLC/RI. L-ascorbic acid supplemented to some children's drinks was analysed by HPLC-NH2/UV. Protein analysis was performed by Experion microfluidic electrophoresis system (BioRad) and protein concentration was verified by colorimetric method. In general, the highest content of phenolic compounds was shown in tea bags when compared with instant granular beverages that contain mostly added simple carbohydrates. Using contact microbial tests proved immediately after opening and also during manipulation with tea boxes was verified that analysed baby teas are in agreement with strict hygiene requirements for baby beverages.
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Analysis of phenolics in different kinds of coffee
Jašková, Martina ; Halienová, Andrea (referee) ; Márová, Ivana (advisor)
The aim of this bachelor thesis was examining the quantity of biologically significant substances in selected types of roasted ground coffee, instant coffee and coffee extract. The particular kinds of coffee differed in technological processing, degree of roasting and composition. In the practical part total phenolics and flavonoids by using spectrophotometry methods. The total antioxidant activity in the individual coffee extracts was determined by ABTS method. Qualitative and quantitative analysis of some individual antioxidants and caffeine was performed using RP-HLPC with spectrophotometric detection. As a part of the work sensory analysis of all coffee samples was realized and consumer questionnaires were completed.
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