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Analysis of the specifics of bookkeeping in an agricultural enterprise
JANOUŠKOVÁ, Adéla
The main objective of this thesis is to analyse the specifics of accounting in an agricultural enterprise. The thesis should provide a detailed overview of how an accounting unit keeps accounts mainly in crop and livestock production. A sub-objective is subsidies and their effect on the profit and loss. The information for the literature search has been drawn from scientific literature, publications, books and internet sources. The analysis of accounting specifics for this thesis was prepared in the Agricultural Company Slapy, a.s. The practical part is based on the company's financial statements and accounting software. Horizontal data analysis is used in the thesis to determine the changes that occurred in individual accounts between years. Thanks to vertical analysis, the thesis presents, for example, the percentage shares of individual activities in total sales, with the largest part being plant production. The analysis revealed that crop production is widely used in unfinished production is used extensively in crop production. This account is used for plants whose life cycle does not coincide with the accounting unit's marketing year. Work in progress rec-ords the amount of costs incurred in growing crops in the current accounting period that will not be har-vested until the following accounting period. The company raises cattle mainly for milk production, which is of great importance to the enterprise. If the company had not used the subsidy, its result would have been a loss. The analysis showed that, although the company was using subsidies in 2021 at a comparable level to previous years, it was making a loss.
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Study on impact of selected protein kinase inhibitors on drug resistance mediated by cytochromes P450
Janoušková, Adéla ; Hofman, Jakub (advisor) ; Novotná, Eva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Adéla Janoušková Supervisor: RNDr. Jakub Hofman, Ph.D. Title of diploma thesis: Study on impact of selected protein kinase inhibitors on drug resistance mediated by cytochromes P450 Pharmacokinetic drug resistance often leads to failure of an anticancer therapy. One of the mechanisms is increased efflux of drugs from tumour cells, whereas some studies suggest that increased drug conversion to an inactive metabolite might be another contributing mechanism. The aim of this work was to define the possible role of CYP3A4 and CYP2C8 enzymes in the phenomenon of pharmacokinetic resistance and to investigate the possibility of its modulation by new targeted drugs. In the first part, we used the MTT proliferation method together with HepG2 cells stably transduced with particular human enzymes and demonstrated significant involvement of CYP3A4 in docetaxel resistance. In the following part, we examined the inhibitory effects of four selected tyrosine kinase inhibitors on the CYP3A4 activity in intact cells using a commercial kit. Cobimetinib and dabrafenib showed significant inhibitory activity, while osimertinib and brivanib did not. In the final part, we demonstrated the ability of the first two...
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Study on impact of selected protein kinase inhibitors on drug resistance mediated by cytochromes P450
Janoušková, Adéla ; Hofman, Jakub (advisor) ; Novotná, Eva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Adéla Janoušková Supervisor: RNDr. Jakub Hofman, Ph.D. Title of diploma thesis: Study on impact of selected protein kinase inhibitors on drug resistance mediated by cytochromes P450 Pharmacokinetic drug resistance often leads to failure of an anticancer therapy. One of the mechanisms is increased efflux of drugs from tumour cells, whereas some studies suggest that increased drug conversion to an inactive metabolite might be another contributing mechanism. The aim of this work was to define the possible role of CYP3A4 and CYP2C8 enzymes in the phenomenon of pharmacokinetic resistance and to investigate the possibility of its modulation by new targeted drugs. In the first part, we used the MTT proliferation method together with HepG2 cells stably transduced with particular human enzymes and demonstrated significant involvement of CYP3A4 in docetaxel resistance. In the following part, we examined the inhibitory effects of four selected tyrosine kinase inhibitors on the CYP3A4 activity in intact cells using a commercial kit. Cobimetinib and dabrafenib showed significant inhibitory activity, while osimertinib and brivanib did not. In the final part, we demonstrated the ability of the first two...
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