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Release of bisphenol A and its analogues from dental restorative materials
Tichý, Antonín ; Bradna, Pavel (advisor) ; Izakovičová Hollá, Lydie (referee) ; Suchánek, Jakub (referee)
Bisphenol A (BPA) is an endocrine disruptor that may be released from dental polymeric materials. The aim of this study was to examine the kinetics of the long-term release of BPA and its analogues from restorative materials (composites, hybrid glass ionomer cements) and polycarbonates used in dentistry. Extracts in ethanol and artificial saliva were collected after 1-260 days for restorative materials and after 1-84 days for polycarbonates. All extracts were analyzed by ultra high performance liquid chromatography-tandem mass spectrometry. BPA was detected in all tested materials, while its analogues were not found. The release of BPA from all tested materials was highest during the first day, followed by a significant decrease for all materials and a complete stop for the "BPA-free" composites. Among restorative materials, the highest amounts of BPA were released from composites with BPA-based monomers, followed by hybrid glass ionomer cements. The least BPA was released from "BPA-free" composites. Shorter irradiation time did not significantly affect the total amount of BPA released from restorative materials, but it significantly increased the release of BPA during the first day. The release of BPA from polycarbonates was significantly higher than from restorative materials. Given the current...
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Periopathogens and systemic markers in etiology and diagnostics of periodontal and cardiovascular diseases.
Myšák, Jaroslav ; Foltán, René (advisor) ; Izakovičová Hollá, Lydie (referee) ; Markovská, Neda (referee)
Periodontitis is a disease primarily affecting tooth attachment, i.e. concerning oral cavity, however, its connection to a number of systemic diseases is apparent nowadays. Oral microorganisms and their interaction with the immune system of their host play a significant part in the etiology of this multifactorial disease. Rapid development of DNA-based diagnostic methods in the last 15 years dramatically increased the spectrum of identified oral microorganisms and promoted understanding of how particular taxons correlate with periodontal health or disease. Moreover, next generation sequencing methods also bring new possibilities to study the relationship between periodontitis and other diseases such as diabetes mellitus, cardiovascular diseases (CVD), stroke, lung infection or kidney diseases, etc. The presented work focuses on the use of sequencing methods to compare the taxonomic composition of microbiomes within oral cavity and the tissues of aortic valves in patients suffering from CVD. The characterisation of taxonomic composition of microbiome in the analysed tissues was performed using the method of 454 pyrosequencing of variable region IV-V of the bacterial 16S rDNA. The present taxons were determined by comparing the obtained sequences with the Human Oral Microbiome Database. The common...
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An anthropological study of the eruption of permanent teeth
Šindelářová, Romana ; Broukal, Zdeněk (advisor) ; Špidlen, Miloš (referee) ; Izakovičová Hollá, Lydie (referee)
The introduction to the dissertation is devoted to a brief overview of the development and emergence of teeth and the characteristics of the internal and external influences that affect the emergence. In addition to the influence of physical constitution on permanent teeth emergence, other factors such as socioeconomic influences, premature birth, premature loss of deciduous teeth, or the influence of craniofacial morphology are described. The term biological age and the possibilities of its determination were also discussed. In research part we carried out research into permanent tooth emergence in Czech children aged 4 to 15, as a knowledge of the timing and sequence of the emergence of permanent is important for dental prevention treatment planning, particularly in orthodontics and pediatric dentistry and also is essential in forensic anthropology for the estimation of an individual's age. We also focused on the evaluation of the differences in the time of emergence of the contralateral and opposing permanent teeth. Attention was also paid to some important internal and external factors that affect the time of emergence of the permanent dentition. Our results were compared with the conclusions of similar studies conducted throughout the world. Key words: permanent teeth, emergence time,...
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