|
|
Separation of tocopherols using HPLC technique
Hutníková, Michaela ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Michaela Hutníková Supervisor: assoc. prof. RNDr. Lenka Kujovská Krčmová Ph.D. Title of diploma thesis: Separation of tocopherols using HPLC technique Vitamin E represents eight related compounds α, β, γ, δ-tocopherols (saturated phytyl side chain) and α, β, γ, δ-tocotrienols (unsaturated phytyl side chain). α-tocopherol was the most studied isomer in the past and its anti-inflammation and proliferative effect was described. Therefore most of cancer prevention trials have been focused on α-tocopherol. Present research studies have described the important role of γ and δ-tocopherols in cell proliferation, anti-inflammation and tumor burden. Also it has been demonstrated that γ-tocopherol inhibits colon, prostate, mammary and lung tumor genesis in animal models, suggesting to have a high potential in the prevention of human cancer. In this study the novel sensitive method for analysis of α, β, γ, δ-tocopherols was developed using the High Performance Liquid Chromatography (HPLC) technique. All the measurements were carried out on the chromatographic system HPLC Prominence LC 20, Shimadzu (Kyoto, Japan). During the method development core-shell columns-Kinetex (Torrance, USA) with different...
|
guest ::
RSS feed.