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Testing of Chromatographic Qualities of Alternative Stationary Phases
Havlátová, Michaela ; Šatínský, Dalibor (advisor) ; Nováková, Lucie (referee)
1 Abstract Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Michaela Havlátová Consultant: Doc. RNDr. Dalibor Šatínský, PhD. Diploma Thesis Title: Testing of Chromatographic Qualities of Alternative Stationary Phases Chromatographic qualities of HS F5 column were tested at selected groups of substances. For testing there were chosen substances from the following groups: beta-sympatholytics (acebutolol, bopindolol), neuroleptics (thioridazine, perphenazine), local anesthetics (lidocaine, prilocaine), tetracyclins (oxytetracyclin, minocyclin), methylxanthine derivates (caffeine, theophylline) and aniline. During the analysis the retention of the tested substances was investigated as influenced by the character of the mobile phase. Some of the substances showed U-shaped retention. Three series of analysis were carried out at each tested group. In analysis number 1 acetonitrile - water was used as the mobile phase, in analysis number 2 the mobile phase was acetonitrile - phosphoric acid 0.085%, and in analysis number 3 it was acetonitrile - acetate buffer solution pH 7. In each analysis the acetonitrile percentage in the mobile phase was changed. In the group of the substances tested the U-shaped retention was shown by beta-sympatholytic...
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Monitoring of Retention "U-profiles" of Selected Substances on Pentafluorophenyl Stationary Phase
Havlátová, Michaela ; Šatínský, Dalibor (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Analytical Chemistry Department Candidate: Mgr. Michaela Havlátová Consultant: Doc. RNDr. Dalibor Šatínský, PhD. Rigorous Thesis Title: Retention "U" profiles testing of selected substances on pentafluorophenyl stationary phase Retention "U" profiles were examined on pentafluorophenyl stationary phase at selected substances. For testing there were chosen substances from the group of tetracycline antibiotics (oxytetracycline, minocycline), neuroleptics (thioridazine, perphenazine), local anesthetics (lidocaine, prilocaine), methylxanthine derivates (caffeine, theophylline), ß-sympatholytics (acebutolol, bopindolol) and aniline. During the analysis the retention profiles of the tested substances were observed as influenced by the character of methanol mobile phase. Three analysis series were carried out at all of the tested substances. In analysis series number 1 the mobile phase methanol - water was used. For analysis series number 2 it was the mobile phase methanol - phosphoric acid 0,085% and for analysis series number 3 it was the mobile phase methanol - acetate buffer solution pH 7. In each analysis the methanol percentage in the mobile phase was changed. In the group of the tested substances the U-shaped retention profile was...
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Monitoring of Retention "U-profiles" of Selected Substances on Pentafluorophenyl Stationary Phase
Havlátová, Michaela ; Šatínský, Dalibor (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Analytical Chemistry Department Candidate: Mgr. Michaela Havlátová Consultant: Doc. RNDr. Dalibor Šatínský, PhD. Rigorous Thesis Title: Retention "U" profiles testing of selected substances on pentafluorophenyl stationary phase Retention "U" profiles were examined on pentafluorophenyl stationary phase at selected substances. For testing there were chosen substances from the group of tetracycline antibiotics (oxytetracycline, minocycline), neuroleptics (thioridazine, perphenazine), local anesthetics (lidocaine, prilocaine), methylxanthine derivates (caffeine, theophylline), ß-sympatholytics (acebutolol, bopindolol) and aniline. During the analysis the retention profiles of the tested substances were observed as influenced by the character of methanol mobile phase. Three analysis series were carried out at all of the tested substances. In analysis series number 1 the mobile phase methanol - water was used. For analysis series number 2 it was the mobile phase methanol - phosphoric acid 0,085% and for analysis series number 3 it was the mobile phase methanol - acetate buffer solution pH 7. In each analysis the methanol percentage in the mobile phase was changed. In the group of the tested substances the U-shaped retention profile was...
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Testing of Chromatographic Qualities of Alternative Stationary Phases
Havlátová, Michaela ; Šatínský, Dalibor (advisor) ; Nováková, Lucie (referee)
1 Abstract Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Michaela Havlátová Consultant: Doc. RNDr. Dalibor Šatínský, PhD. Diploma Thesis Title: Testing of Chromatographic Qualities of Alternative Stationary Phases Chromatographic qualities of HS F5 column were tested at selected groups of substances. For testing there were chosen substances from the following groups: beta-sympatholytics (acebutolol, bopindolol), neuroleptics (thioridazine, perphenazine), local anesthetics (lidocaine, prilocaine), tetracyclins (oxytetracyclin, minocyclin), methylxanthine derivates (caffeine, theophylline) and aniline. During the analysis the retention of the tested substances was investigated as influenced by the character of the mobile phase. Some of the substances showed U-shaped retention. Three series of analysis were carried out at each tested group. In analysis number 1 acetonitrile - water was used as the mobile phase, in analysis number 2 the mobile phase was acetonitrile - phosphoric acid 0.085%, and in analysis number 3 it was acetonitrile - acetate buffer solution pH 7. In each analysis the acetonitrile percentage in the mobile phase was changed. In the group of the substances tested the U-shaped retention was shown by beta-sympatholytic...
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