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Study of novel phthalocyanine photosensitizers on cellular level
Halašková, Marie ; Macháček, Miloslav (advisor) ; Pejchal, Jaroslav (referee) ; Hofman, Jakub (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Marie Halašková Supervisor: RNDr. Miloslav Macháček, Ph.D. Title of disertation thesis: Study of novel phthalocyanine photosensitizers on cellular level Photodynamic therapy (PDT) is a clinically approved treatment modality that experienced great progress over the last two decades and that has been used in the treatment of several disorders, including age-related macular degeneration, psoriasis and certain cancers. PDT is based on the combination of three main components: light-sensitive drug (known as a photosensitizer, PS), light of an appropriate wavelength, and oxygen. The light-activated PS reacts with molecular oxygen to produce reactive oxygen species, which can interact with cellular components in a biological environment and trigger a cascade of reactions causing destruction of tumor cells, tumor vasculature and induction of a local inflammatory reaction. The result is a disruption of cell homeostasis leading to the cell death. Like all the novel treatment modalities, further research is still needed including the development and optimization of PS synthesis in order to increase the efficiency of therapy and minimalize side effects. In this project, we decided to carry out an...
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Biological evaluation of photodynamic activity of amphiphilic anionic photosensitizers
Halašková, Marie ; Macháček, Miloslav (advisor) ; Šimůnek, Tomáš (referee)
1 ABSTRACT Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Marie Halašková Supervisor: RNDr. Miloslav Macháček, Ph.D. Title of diploma thesis: Biological evaluation of photodynamic activity of amphiphilic anionic photosensitizers Tumor diseases are a global health problem and one of the most common causes of death in developed countries. Therefore, considerable effort is devoted to research and develop novel anticancer drugs. Common conventional therapies, such as chemotherapy, radiotherapy, surgery, or their combination, are often associated with severe side effects and high treatment costs. Photodynamic therapy (PDT) is therefore being increasingly recognized as an alternative treatment modality for the treatment of many malignant and non-malignant conditions. PDT is a clinically approved non-invasive treatment which consists of two relatively simple procedures: administration of a photosensitive drug and subsequent radiation of the tumor by light to activate the photosensitizer (PS). The activated photosensitizer reacts with molecular oxygen to form highly reactive oxygen species (ROS). This creates a state of oxidative stress in the tumor which leads to tumor cell death, tumor (micro)vasculature shutdown and induction of anti-tumor immune...
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Biological evaluation of photodynamic activity of amphiphilic anionic photosensitizers
Halašková, Marie ; Macháček, Miloslav (advisor) ; Šimůnek, Tomáš (referee)
1 ABSTRACT Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Marie Halašková Supervisor: RNDr. Miloslav Macháček, Ph.D. Title of diploma thesis: Biological evaluation of photodynamic activity of amphiphilic anionic photosensitizers Tumor diseases are a global health problem and one of the most common causes of death in developed countries. Therefore, considerable effort is devoted to research and develop novel anticancer drugs. Common conventional therapies, such as chemotherapy, radiotherapy, surgery, or their combination, are often associated with severe side effects and high treatment costs. Photodynamic therapy (PDT) is therefore being increasingly recognized as an alternative treatment modality for the treatment of many malignant and non-malignant conditions. PDT is a clinically approved non-invasive treatment which consists of two relatively simple procedures: administration of a photosensitive drug and subsequent radiation of the tumor by light to activate the photosensitizer (PS). The activated photosensitizer reacts with molecular oxygen to form highly reactive oxygen species (ROS). This creates a state of oxidative stress in the tumor which leads to tumor cell death, tumor (micro)vasculature shutdown and induction of anti-tumor immune...
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