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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Genetic engineering of \kur{psbA} gene in \kur{Nicotiana tabacum}
 HUCKOVÁ, Dagmar
Transformation vector with mutated psbA gene and selective aadA gene was created and transferred into Nicotiana tabacum living cells using Biolistic bombardment. Due to homologous recombination, transformed plant lineage carrying D1-A209, D1-C-212 instead of D1-S209, D1-S212 in D1 protein in PS II was obtained. Seeds from transformed plant were harvested and homoplasmy of the first generation was tested. These mutations caused higher thermostability in Synechocystis sp. PCC6803 so the transformed plant is expected to be the first step in the study of PS II thermostability in higher plants.
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Genetic structure of European grayling (\kur{Thymallus thymallus}) populations in the Czech rivers affected by fishery management
 HUCKOVÁ, Dagmar
Populations of freshwater fish Thymallus thymallus are considerably affected by intensive human mediated stock transfer in the rivers of Central Europe. These activities cause exogenous genetic drift among populations from geographical isolated hydrological units. Genetic structure of 369 T. thymallus individuals of 21 populations from three river basins of the Czech Republic was studied based on analysing of both mtDNA (control region) and nuclear DNA (7 microsatellites loci) markers and the results were compared with similar studies from European region.
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