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Cytotoxicity of mycotoxins in vitro methods using CHO-K1 cells
Franzová, Pavla ; Vopršalová, Marie (advisor) ; Herink, Josef (referee)
The purpose of this study was to determine the capacity of three Fusarium mycotoxins: BEA, DON, T-2 toxin, to induce cytotoxicity using the mammalian CHO-K1 cells by the NR assay. CHO-K1 cells were exposed to several concetrations of DON, BEA and T-2 toxin and several incubation times. The extent of cell injury was assessed by NR uptake assay, after an incubation period of 24, 48 and 72h. Moreover, the viability of CHO-K1 cells was measured in the presence of a mixture of two or three of the mycotoxins. Significant differences were observed between the compounds tested. All toxins, DON, BEA and T-2 toxin, tested individually diminish cell viability. Individual mycotoxins reduce viability in increasing order: BEA<DON<T-2 toxin. Our results show that CHO-K1 cells are extremely sensitive to T-2 toxin. T-2 toxin exhibited the most cytotoxic response against the cell line tested. Results obtained indicate that CHO-K1 cell line, exhibited a time and concentration- dependent cytotoxicity. Our results demonstrated that the T-2 toxin was found to be more cytotoxic during the exposure period, which was totally in agreement with the data previously published. The aim of this study was also to evaluate the cytotoxicity of low concentrations of mycotoxin combinations on CHO-K1 cells. We have studied the...
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Cytotoxicity of mycotoxins in vitro methods using CHO-K1 cells
Franzová, Pavla ; Herink, Josef (referee) ; Vopršalová, Marie (advisor)
The purpose of this study was to determine the capacity of three Fusarium mycotoxins: BEA, DON, T-2 toxin, to induce cytotoxicity using the mammalian CHO-K1 cells by the NR assay. CHO-K1 cells were exposed to several concetrations of DON, BEA and T-2 toxin and several incubation times. The extent of cell injury was assessed by NR uptake assay, after an incubation period of 24, 48 and 72h. Moreover, the viability of CHO-K1 cells was measured in the presence of a mixture of two or three of the mycotoxins. Significant differences were observed between the compounds tested. All toxins, DON, BEA and T-2 toxin, tested individually diminish cell viability. Individual mycotoxins reduce viability in increasing order: BEA<DON<T-2 toxin. Our results show that CHO-K1 cells are extremely sensitive to T-2 toxin. T-2 toxin exhibited the most cytotoxic response against the cell line tested. Results obtained indicate that CHO-K1 cell line, exhibited a time and concentration- dependent cytotoxicity. Our results demonstrated that the T-2 toxin was found to be more cytotoxic during the exposure period, which was totally in agreement with the data previously published. The aim of this study was also to evaluate the cytotoxicity of low concentrations of mycotoxin combinations on CHO-K1 cells. We have studied the...
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Study of factors influencing weight of oral drops
Franzová, Pavla ; Šklubalová, Zdeňka (advisor) ; Řehula, Milan (referee)
10 SUMMARY The factors influencing weight of peroral drops were investigated in this dissertation. During this work six commercial available plastic dropper tips for peroral drops and model mixtures of hydrophil solvents were used. It was determined that the most suitable mixture for testing dropper charakteristics (depending on design of dropper tip) was the mixture of five parts of 96% ethanol and ninetyfive parts of purified water or the mixtureof sixty parts of 85% glycerol and forty parts of purified water. The weight of drop was influenced by the diameter of outer orifice of dropper tip. The final weight of drop is also affected by the other charakteristics - inner diameter of dropper part, inner aperture diameter of dropper capilary, design of dropper tip, design of compensatory capilary. The drop weight of mixture of water with glycerol increased with decreasing density of mixture. The reduction of dropper angle from 90ř (vertical) to 45ř (transversal) lead to decisive reduction of drop weight. The uncontrolled wettion of dropper surface by the liquid was observed in some cases. On the base of optimal dispending of peroral drops we should recommend the producer following: there should be the instruction about the right holding of dropper bottle in upright position (upside-down, 90ř vertical)
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