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National Repository of Grey Literature 3 records found  Search took 0.02 seconds. 
Determination of pharmaceuticaly important substances using High Performance Liquid Chromatography with Mass Spectrometry detection
 Barrett, Blanka
High performance liquid chromatography (HPLC) in combination with Mass Spectrometry (MS) is frequently used for the determination of drugs in human plasma and it belongs to group of methods that enable new and modern modifications. Highly sensitive methods that do not require time consuming sample pre-treatment are desired for pharmacokinetic (PK) studies. Double MS (MS/MS) detection provides drugs identification and high sensitivity for quantitative determination. It effectively eliminates interferences from endogenous impurities and guarantees method selectivity at the same time. PK studies determine pharmacokinetic characteristics of particular drug that are then used to assess the bioequivalence of determined drug versus other chosen drug of the same nature. The PK parameters are calculated from the plasma concentrations of the drug determined by a validated method. The aim of my thesis was to show the use of HPLC-MS/MS methods in pharmacokinetic studies. All developed methods were successfully employed in the pharmacokinetic studies to assess the pharmacokinetic parameters. A validated, highly sensitive, and selective HPLC method with MS-MS detection has been developed for quantitative determination of azithromycin (AZI) in human Na2EDTA plasma. Roxithromycin (ROX) was used as internal...
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Determination of pharmaceuticaly important substances using High Performance Liquid Chromatography with Mass Spectrometry detection
 Barrett, Blanka
High performance liquid chromatography (HPLC) in combination with Mass Spectrometry (MS) is frequently used for the determination of drugs in human plasma and it belongs to group of methods that enable new and modern modifications. Highly sensitive methods that do not require time consuming sample pre-treatment are desired for pharmacokinetic (PK) studies. Double MS (MS/MS) detection provides drugs identification and high sensitivity for quantitative determination. It effectively eliminates interferences from endogenous impurities and guarantees method selectivity at the same time. PK studies determine pharmacokinetic characteristics of particular drug that are then used to assess the bioequivalence of determined drug versus other chosen drug of the same nature. The PK parameters are calculated from the plasma concentrations of the drug determined by a validated method. The aim of my thesis was to show the use of HPLC-MS/MS methods in pharmacokinetic studies. All developed methods were successfully employed in the pharmacokinetic studies to assess the pharmacokinetic parameters. A validated, highly sensitive, and selective HPLC method with MS-MS detection has been developed for quantitative determination of azithromycin (AZI) in human Na2EDTA plasma. Roxithromycin (ROX) was used as internal...
Detailed record
Determination of pharmaceuticaly important substances using High Performance Liquid Chromatography with Mass Spectrometry detection
 Barrett, Blanka ; Jelínek, Ivan (advisor) ; Feltl, Ladislav (referee) ; Cvačka, Josef (referee)
L, L- L. l_ t__ L- INTRoDacrtoN High peďommce liquid chromatography (HPLC) is the most common method used in commercial malÍlcal laboratories. HPLC in combination with Mss speďIometry (MS) is frequmtly used for the detemination of drugs in hmm plasma md it belongs to gÍoup of methods that enable new md modem modifications. That is why following new trmds, which broadm the field of application of these methods. is so importmt. HPLC with Ms detection (HPLc-Ms) satisfy demmd for automation of detemination of smples from complex matrixes, which lead to elimination of humm enor, decreme of *penditure of maly,tical process md finally hildling potentially dmgerous smples by laboratory stďl Hrghly sensitive methods that do not require time consuming smple pre-teatment ue desired for phmacokinetic (PK) studies. Double MS (Ms/Ms) detection provides drugs identification md hgh sensitivity fol qumtitative detemination- It effectively eliminates inteďerences from endogenous impurities md guďmtees method selectivity at the sme time- Phmacokinetic (PK) studies detemine phmacokinetic chďactgistics of particulr drug that ile thm used to assess the bioequivalence of detemined drug vqsus othď chosm dÍug of the same nafurc' The PK paÍmetqs ďe calculated from the plmma concentrations of the drug detmined by a validated method'...
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