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HPLC analysis of drugs III
Blažková, Petra ; Sochor, Jaroslav (advisor) ; Mokrý, Milan (referee)
ANALYTICAL EVALUATION OF ACTIVE SUBSTANCE BY LIQUID CHROMATOGRAPHY II. Diploma thesis Petra Blažková Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control, Heyrovského 1203, Hradec Králové The subject of this diploma thesis is a determination of active substances by liquid chromatography. In particular it deals with an evaluation of phenobarbital in biological matrix. Solid phase microextraction (SPME) and high performance liquid chromatography (HPLC) with UV detector (218 nm) and reversed phase C18 comlumn were used. The mobile phase consisted of methanol and phosphate buffer in a ratio of 50:50. The flow rate was set to 0,4 ml/min. 20 µl of sample solution was injected on the chromatographic column. Phenobarbital was dissolved in a mixture of methanol and buffer in a ratio of 80:20. For the SPME extraction from water solution was used carbowax/TPR fibre. The analyte was desorbed into methanol. The extraction time was 20 minutes. Phenobarbital extraction from rabbit plasma was performed in the same way, but PDMS/DVB fibre instead of carbowax/TPR fibre was used. Phenobarbital in rabbit plasma was quantified by means of calibration curve.
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Analysis of Phenobarbital in Blood of SPME Method Coupled off-line with HPLC
Blažková, Petra ; Sochor, Jaroslav (advisor) ; Mokrý, Milan (referee)
Charles University in Prague, Pharmaceutical Faculty in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Candidate: Mgr. Petra Blažková Consultant: Doc. RNDr. Jaroslav Sochor, Csc. ANALYSIS OF PHENOBARBITAL IN BLOOD OF SPME METHOD COUPLED OFF-LINE WITH HPLC The purpose of the work presented in this thesis was an analytical evaluation of active ingredients using High Performance Liquid Chromatography. The core of the work was an evaluation of phenobarbital level in biological material using Solid Phase Microextraction method in off-line conjunction with HPLC. The wavelength used for detection was 218 nm. C18 HPLC column was used to perform the analysis. Mobile phase was a mixture of 50 parts of methanol and 50 parts of water. The flow rate was set to 0,6 ml/min. Sample amount was 20 µl. A mixture of 80 parts of methanol and 20 parts of a buffer was used as a solvent to dissolve phenobarbital. PDMS/DVB fibre was used to extract the drug from a blood sample by SPME. Sorption and desorption time was 20 minutes. The drug was desorbed into methanol. A calibration curve was created for the quantitative analysis of phenobarbital in rabbit blood. The conditions under which the experiment was undertaken were validated.
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Processing of non-musical themes in compositions for keyboard instruments
Živná, Tereza ; MRÁZKOVÁ, Giedre (advisor) ; BLAŽKOVÁ ŽĎÁRSKÁ, Petra (referee)
The bachelor thesis is focused for processing programme music in keyboard literature in second half of the seventeenth century in Germany with a focus on Johann Kuhnau and his Biblical sonatas. The aim of this work is outline idea perception of music in second half of the seventeenth century in Germany and focusing non musical themes, which were widely used in Baroque. I focused on the analysis of individual Johann Kuhnau´s Biblical sonatas and on combining the biblical story with music. The knowledge for me is the depth of this connection and the detailed thinking of baroque composers.
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Alpha-1-antitrypsin deficiency analysis using real-time PCR
Blažková, Petra ; Beránek, Martin (advisor) ; Šimůnek, Tomáš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Petra Blažková Supervisor: Doc. PharmDr. Martin Beránek Ph.D. Title of diploma thesis: Alpha-1-antitrypsin deficiency analysis using real-time PCR This diploma thesis focuses on the validation of the method PCR in real-time (real-time PCR) to investigate the Z and S mutations in the gene SERPINA1, located at the long arm of chromosome 14 (14q32.13) and provides instructions for making a protein named alpha-1- antitrypsin (A1AT). A1AT is a serine protease inhibitor that protects tissues from degradation by neutrophil elastase. Deficiency, which is most commonly caused by these mutations, can cause children and adult's liver and lung disease. A method of real-time PCR was applied after successful validation to a set of 46 clinical samples of DNA extracted from blood samples and 30 samples of the DNA from cells of the buccal mucosa. DNA isolation was performed by kit extraction QIAamp ® DNA Mini Kit by QIAGEN. I used a set of primers and hybridization probes according to Snyder (2006) for genotyping. Melting curve analysis was carried out in the thermocycler LightCycler 1.2. Results of DNA samples obtained from blood were compared with the results obtained through an accredited method...
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Šprechtíme campaign in years 2011-2012
Blažková, Petra ; Halada, Jan (advisor) ; Dolanská, Nora (referee)
This thesis deals with a description and analysis of communication activities, which took place during the Šprechtíme campaign in years 2011 - 2012. Campaign has been carried out by the Embassy of the Federal Republic of Germany together with the Embassy of Austria and other partners. The purpose of the campaign is to support the teaching of German language in the Czech Republic. The main advantage of knowledge of the language, according to Embassies, is a possibility to easily get a job, because the German and Austrian companies in the Czech Republic created nearly 125,000 jobs. Furthermore, it is easier to communicate on vacation in the Alps. Mapping communication activities is accompanied by partial evaluations, further evaluation of the campaign as a whole. The last part is devoted to the recommendation of the author, which activities should be changed or strengthened.
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Analysis of Phenobarbital in Blood of SPME Method Coupled off-line with HPLC
Blažková, Petra ; Sochor, Jaroslav (advisor) ; Mokrý, Milan (referee)
Charles University in Prague, Pharmaceutical Faculty in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Candidate: Mgr. Petra Blažková Consultant: Doc. RNDr. Jaroslav Sochor, Csc. ANALYSIS OF PHENOBARBITAL IN BLOOD OF SPME METHOD COUPLED OFF-LINE WITH HPLC The purpose of the work presented in this thesis was an analytical evaluation of active ingredients using High Performance Liquid Chromatography. The core of the work was an evaluation of phenobarbital level in biological material using Solid Phase Microextraction method in off-line conjunction with HPLC. The wavelength used for detection was 218 nm. C18 HPLC column was used to perform the analysis. Mobile phase was a mixture of 50 parts of methanol and 50 parts of water. The flow rate was set to 0,6 ml/min. Sample amount was 20 µl. A mixture of 80 parts of methanol and 20 parts of a buffer was used as a solvent to dissolve phenobarbital. PDMS/DVB fibre was used to extract the drug from a blood sample by SPME. Sorption and desorption time was 20 minutes. The drug was desorbed into methanol. A calibration curve was created for the quantitative analysis of phenobarbital in rabbit blood. The conditions under which the experiment was undertaken were validated.
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HPLC analysis of drugs III
Blažková, Petra ; Mokrý, Milan (referee) ; Sochor, Jaroslav (advisor)
ANALYTICAL EVALUATION OF ACTIVE SUBSTANCE BY LIQUID CHROMATOGRAPHY II. Diploma thesis Petra Blažková Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control, Heyrovského 1203, Hradec Králové The subject of this diploma thesis is a determination of active substances by liquid chromatography. In particular it deals with an evaluation of phenobarbital in biological matrix. Solid phase microextraction (SPME) and high performance liquid chromatography (HPLC) with UV detector (218 nm) and reversed phase C18 comlumn were used. The mobile phase consisted of methanol and phosphate buffer in a ratio of 50:50. The flow rate was set to 0,4 ml/min. 20 µl of sample solution was injected on the chromatographic column. Phenobarbital was dissolved in a mixture of methanol and buffer in a ratio of 80:20. For the SPME extraction from water solution was used carbowax/TPR fibre. The analyte was desorbed into methanol. The extraction time was 20 minutes. Phenobarbital extraction from rabbit plasma was performed in the same way, but PDMS/DVB fibre instead of carbowax/TPR fibre was used. Phenobarbital in rabbit plasma was quantified by means of calibration curve.
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Ochrana hospodářské soutěže v podmínkách ČR se zaměřením na oblast telekomunikací
Blažková, Petra
The diploma thesis deals with the protection of competition in the Czech Republic with a focus on telecommunications. The literature research describes in imperfectly competitive markets, competition, telecommunications sector and forms of his regulation and legislation in the Czech Republic and the European Union. The own part of the thesis deals with the analysis of the competitive environment of the telecommunications market and regulator measures in the Czech Republic.
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