|
| |
|
|
Delivery of genomes of nonenveloped DNA viruses into the cell nucleus
Bílková, Eva ; Forstová, Jitka (advisor) ; Hatáková, Ladislava (referee)
The majority of DNA viruses have to deliver their genome to the cell nucleus, which provi- des factors required for their replication and transcription. This work is focused on this proces of small nonenveloped DNA viruses. It describes delivery of the adenovirus, parvovirus, pa- pillomavirus and polyomavirus genomes into the cell nucleus. These viruses are endocyted by the cell and travel to the enveloped compartments. Viral particle undergoes changes afffected by surrounding environment and activity of cellular enzymes, which results in its escape from enveloped vesicle mediated by the viral proteins. Some viruses use direct interactions with cytos- keletal transport components for travelling to the cell nucleus. In most cases, viral DNA enters cell nucleus via nuclear pore complex, although the evidence of alternative mechanisms exists as well. This work focuses on early phases of the life cycle of the selected viruses and the nucleus targeting of their genomes. Understanding the mechanisms of viral DNA nuclear import may contribute to discovery of new anti-viral therapies.
|
|
|
Studies of properties of the minor structural proteins of the Murine polyomavirus
Bílková, Eva ; Forstová, Jitka (advisor) ; Němečková, Šárka (referee)
Murine polyomavirus (MPyV) is a member of the Polyomaviridae family. Its capsid is composed of the major capsid protein, VP1, and the minor proteins, VP2 and VP3. The minor capsid proteins probably assure delivery of the viral genome through the endoplasmic reticulum membrane to the nucleus during early phase of infection. However, precise mechanism is not known. Expression plasmids encoding mutated VP2 or VP3 fused with EGFP have been constructed to study the interaction of VP2 and VP3 with membranes. The mutated proteins have deletions in the predicted hydrophobic domains. In this thesis, cell localisation of mutated proteins was followed. The study revealed that the hydrophobic domain 2 is the most important for association of VP2 and VP3 with membranes, while domains 1 and 3 are rather expendable. Further, nature of VP2 and VP3 isoforms has been studied. Isoforms with different electrophoretic mobility were separated on SDS-PAGE. Consequent mass spectrometry analysis showed that they differ in deamidation of asparagine, present at both minor proteins (position 253 of VP2 and 137 of VP3). Previously, acetylation of VP3 N-terminal alanine has been identified. To elucidate the function of these modifications, mutated viruses were constructed with substitution of these amino acids. Pilot...
|
|
|
Delivery of genomes of nonenveloped DNA viruses into the cell nucleus
Bílková, Eva ; Forstová, Jitka (advisor) ; Hatáková, Ladislava (referee)
The majority of DNA viruses have to deliver their genome to the cell nucleus, which provi- des factors required for their replication and transcription. This work is focused on this proces of small nonenveloped DNA viruses. It describes delivery of the adenovirus, parvovirus, pa- pillomavirus and polyomavirus genomes into the cell nucleus. These viruses are endocyted by the cell and travel to the enveloped compartments. Viral particle undergoes changes afffected by surrounding environment and activity of cellular enzymes, which results in its escape from enveloped vesicle mediated by the viral proteins. Some viruses use direct interactions with cytos- keletal transport components for travelling to the cell nucleus. In most cases, viral DNA enters cell nucleus via nuclear pore complex, although the evidence of alternative mechanisms exists as well. This work focuses on early phases of the life cycle of the selected viruses and the nucleus targeting of their genomes. Understanding the mechanisms of viral DNA nuclear import may contribute to discovery of new anti-viral therapies.
|
|
|
Development of children's drawings from 3 and 5 grade class
BÍLKOVÁ, Eva
This thesis focuses on the comparison of two different primary school classes, one third grade and one fifth grade, in the area of self-expression through drawing. The theoretical part considers children of younger school age at different stages, followed by further general information about artistic expression and developmental stages of drawing human figures. The practical part of the thesis shows the results of drawing figures, a comparison of human figures, and the standard of drawing such figures in third and fifth grade classes. The results are assessed on the basis of criteria that are laid down in the thesis
|
|
|
The financial statements in the joint stock company
Bílková, Eva ; Müllerová, Libuše (advisor) ; Charvát, Jan (referee)
The aim of this thesis is to describe the process of financial statements in the joint stock company in terms of Czech legislation. The thesis is divided into two parts, a theoretical part discusses the operations preceding the closing of accounting books, the closing ledger accounts and financial statements with focus on respecting the true and fair view of the company and subsequent accounting duties related to financial statements. The practical part follows analysing the steps of the financial statements of particular joint stock company. The whole text is based on real data and demonstrates some situations characterised in the theoretical part.
|
guest ::
RSS feed.