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	Preparation and characterization of modified viral particles derived from mouse polyomavirus for the transport of genes to increase the efficiency of transduction Škvára, Petr ; Španielová, Hana (advisor) ; Sýkora, Michal (referee) Viral particles derived from mouse polyomavirus can be potentially used as a delivery system for therapeutic genes and drugs into target cells. This thesis focuses on preparation and characterization of polyomaviral particles that are modified with cell-penetrating peptides in order to increase efficiency of transduction of reporter genes into human cells. Viral particles that are composed of major capsid protein VP1 in combination with minor capsid protein VP2 and minor capsid protein VP3 that is modified with octaarginine, LAH4 peptide or with transduction domain of adenoviral protein VI are analysed in transduction assays. The thesis also provides information about the effect of the modification on encapsidation of heterologous DNA. The results of transduction assays performed with modified particles containing encapsidated luciferase gene revealed that efficiency of transduction did not increase but decreased in comparison with unmodified particles. These findings help to elucidate the role of polyomaviral minor capsid proteins in gene transfer mediated by viral particles and contribute to the design of new strategies for modifications of viral particles derived from mouse polyomavirus for their successful application in nanomedicine. Key words: mouse polyomavirus, pseudovirions, virus-like... Detailed record
	Preparation and characterization of modified viral particles derived from mouse polyomavirus for the transport of genes to increase the efficiency of transduction Škvára, Petr ; Španielová, Hana (advisor) ; Sýkora, Michal (referee) Viral particles derived from mouse polyomavirus can be potentially used as a delivery system for therapeutic genes and drugs into target cells. This thesis focuses on preparation and characterization of polyomaviral particles that are modified with cell-penetrating peptides in order to increase efficiency of transduction of reporter genes into human cells. Viral particles that are composed of major capsid protein VP1 in combination with minor capsid protein VP2 and minor capsid protein VP3 that is modified with octaarginine, LAH4 peptide or with transduction domain of adenoviral protein VI are analysed in transduction assays. The thesis also provides information about the effect of the modification on encapsidation of heterologous DNA. The results of transduction assays performed with modified particles containing encapsidated luciferase gene revealed that efficiency of transduction did not increase but decreased in comparison with unmodified particles. These findings help to elucidate the role of polyomaviral minor capsid proteins in gene transfer mediated by viral particles and contribute to the design of new strategies for modifications of viral particles derived from mouse polyomavirus for their successful application in nanomedicine. Key words: mouse polyomavirus, pseudovirions, virus-like... Detailed record
	Avian polyomaviruses Škvára, Petr ; Fraiberk, Martin (advisor) ; Forman, Martin (referee) Avian polyomaviruses are small, nonenveloped viruses from family Polyomaviridae, which can cause acute infection with high mortality of infected birds. In contrast with other polyomaviruses, avian polyomaviruses do not share similar pathogenesis, because most of them do not cause acute diseases. The main point of this work is to summarize available information about avian polyomaviruses and to compare them to each other. This work also compares avian polyomaviruses with mammalian polyomaviruses. The primary focus is made on genome organization, structure and function of viral proteins, but also on pathogenesis and epidemiology of avian polyomaviruses. In conclusion, even if avian polyomaviruses share common signs with other polyomaviruses, they have different conserved sequences, host specificity and pathogenicity. Summary of current knowledge about avian polyomaviruses could help to understand the importance of this newly established genus in the field of polyomavirus research. Key words: avian polyomaviruses, taxonomy, genome, viral proteins, pathogenesis Detailed record

See also: similar author names
1	Škvára, Pavel

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