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Optimization of the Determination of Septonex in Pharmaceutical Preparations by Capillary Zone Electrophoresis with Use of Organic Solvents as Additives in the Background Electrolyte
Šindelková, Martina ; Polášek, Miroslav (advisor) ; Pospíšilová, Marie (referee)
OPTIMIZATION OF THE DETERMINATION OF SEPTONEX IN PHARMACEUTICAL PREPARATIONS BY CAPILLARY ZONE ELECTROPHORESIS WITH USE OF ORGANIC SOLVENTS AS ADDITIVES IN THE BACKGROUND ELECTROLYTE A new method of capillary zone electrophoresis with contactless conductivity detection for the determination of septonex in pharmaceutical preparations was devised. Optimal conditions for the separation and determination of septonex were: background electrolyte 30mM MES of pH 7.0 (adjusted with 200mM TRIS) containing 12.5mg/ml of (2- hydroxypropyl)-β-cyclodextrin and 20% (volume), voltage 25kV, temperature 25řC and sample injection for 6 seconds under the pressure of 50mbar. Arginin (200µg/ml) was used as internal standard. The peak of septonex was satisfactorily separated from the peak of internal standard as well as from the EOF. The analysis was carried out in a fused-silica capillary (internal diameter 50μm, total length 75cm and the length to the detector 45cm). The separation took less than 5 minutes and the overall analysis time involving appropriate rinsing of the capillary was less than 13 minutes. The calibration curve was linear in the range 75µg/ml - 400µg/ml of septonex, correlation coefficient r = 0.9997. The LOD was 13,5μg/ml and LOQ was 45μg/ml of septonex. The method is applicable for qualitative as...
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Determination of septonex in pharmaceutical preparations by capillary zone electrophoresis with conductivity detection.
Šindelková, Martina ; Polášek, Miroslav (advisor) ; Pospíšilová, Marie (referee)
DETERMINATION OF SEPTONEX IN PHARMACEUTICAL PREPARATIONS USING CAPILLARY ZONE ELECTROPHORESIS WITH CONTACTLESS CONDUCTIVITY DETECTION A new method of capillary zone electrophoresis with contactless conductivity detection for the determination of septonex in pharmaceutical preparations was devised. Optimal conditions for the separation and determination of septonex were: background electrolyte 30mM MES of pH 7.0 (adjusted with 20mM TRIS) containing 12.5mg/ml of (2- hydroxypropyl)-β-cyclodextrin, voltage 25kV, temperature 25řC and sample injection for 15 seconds under the pressure of 50mbar. N,N-dimethylethanolamine (200µg/ml) was used as internal standard. The peak of septonex was satisfactorily separated from the peak of internal standard as well as from the EOF. The analysis was carried out in a fused-silica capillary (internal diameter 50μm, total length 75cm and the length to the detector 45cm). The separation took less than 4 minutes and the overall analysis time involving appropriate rinsing of the capillary was less than 16 minutes. The calibration curve was linear in the range 75µg/ml - 300µg/ml of septonex, correlation coefficient r = 0.9976. The LOD was 9μg/ml and LOQ was 30μg/ml of septonex. Unsuitable repeatability of peak areas of septonex (caused probably by insufficient elimination of...
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Optimization of the Determination of Septonex in Pharmaceutical Preparations by Capillary Zone Electrophoresis with Use of Organic Solvents as Additives in the Background Electrolyte
Šindelková, Martina ; Pospíšilová, Marie (referee) ; Polášek, Miroslav (advisor)
OPTIMIZATION OF THE DETERMINATION OF SEPTONEX IN PHARMACEUTICAL PREPARATIONS BY CAPILLARY ZONE ELECTROPHORESIS WITH USE OF ORGANIC SOLVENTS AS ADDITIVES IN THE BACKGROUND ELECTROLYTE A new method of capillary zone electrophoresis with contactless conductivity detection for the determination of septonex in pharmaceutical preparations was devised. Optimal conditions for the separation and determination of septonex were: background electrolyte 30mM MES of pH 7.0 (adjusted with 200mM TRIS) containing 12.5mg/ml of (2- hydroxypropyl)-β-cyclodextrin and 20% (volume), voltage 25kV, temperature 25řC and sample injection for 6 seconds under the pressure of 50mbar. Arginin (200µg/ml) was used as internal standard. The peak of septonex was satisfactorily separated from the peak of internal standard as well as from the EOF. The analysis was carried out in a fused-silica capillary (internal diameter 50μm, total length 75cm and the length to the detector 45cm). The separation took less than 5 minutes and the overall analysis time involving appropriate rinsing of the capillary was less than 13 minutes. The calibration curve was linear in the range 75µg/ml - 400µg/ml of septonex, correlation coefficient r = 0.9997. The LOD was 13,5μg/ml and LOQ was 45μg/ml of septonex. The method is applicable for qualitative as...
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Determination of septonex in pharmaceutical preparations by capillary zone electrophoresis with conductivity detection.
Šindelková, Martina ; Polášek, Miroslav (advisor) ; Pospíšilová, Marie (referee)
DETERMINATION OF SEPTONEX IN PHARMACEUTICAL PREPARATIONS USING CAPILLARY ZONE ELECTROPHORESIS WITH CONTACTLESS CONDUCTIVITY DETECTION A new method of capillary zone electrophoresis with contactless conductivity detection for the determination of septonex in pharmaceutical preparations was devised. Optimal conditions for the separation and determination of septonex were: background electrolyte 30mM MES of pH 7.0 (adjusted with 20mM TRIS) containing 12.5mg/ml of (2- hydroxypropyl)-β-cyclodextrin, voltage 25kV, temperature 25řC and sample injection for 15 seconds under the pressure of 50mbar. N,N-dimethylethanolamine (200µg/ml) was used as internal standard. The peak of septonex was satisfactorily separated from the peak of internal standard as well as from the EOF. The analysis was carried out in a fused-silica capillary (internal diameter 50μm, total length 75cm and the length to the detector 45cm). The separation took less than 4 minutes and the overall analysis time involving appropriate rinsing of the capillary was less than 16 minutes. The calibration curve was linear in the range 75µg/ml - 300µg/ml of septonex, correlation coefficient r = 0.9976. The LOD was 9μg/ml and LOQ was 30μg/ml of septonex. Unsuitable repeatability of peak areas of septonex (caused probably by insufficient elimination of...
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