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Leisure activities of second degree primary school pupils from socially disadvantaged background
Šilhavá, Kristýna ; Blažková, Veronika (advisor) ; Hanková, Zdeňka (referee)
The bachelor's thesis on the topic Leisure activities of children from socially disadvantaged backgrounds focuses on how children from socially disadvantaged backgrounds spend their free time. Thesis objective is finding out what activities the second grade students from this environment choose and if and how often they use the free time activity offerings at school or nearby institutions. Last but not least, find out if the free time aktivity offerings of school and surrounding institutions are sufficient, or what other free time activities the pupils would be interested in. The thesis is divided into two chapters, a theoretical and a practical part. The theoretical part defines the concepts of free time, it's functions and the importance of leisure time education. It also deals with social disadvantage and identifying pupils from a socially disadvantaged environment, which was key for the practical part and choosing the research respondents. The second chapter describes the research group and the evaluation of acquired research data. The research part contains the results of a questionnaire survey, describing how children from socially disadvantaged environment spend their free time, which institutions these children visit and what leisure activities they prefer. KEYWORDS Free-time activities,...
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Basics of current antimycobacterial treatment
Šilhavá, Kristýna ; Janďourek, Ondřej (advisor) ; Diepoltová, Adéla (referee)
Charles University, Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Pharmacy Author: Kristýna Šilhavá Supervisor: PharmDr, Ondřej Janďourek, Ph.D. Title of diploma thesis: Basics of current antimycobacterial therapy The aim of the theoretical part of this thesis is to summarize information regarding mycobacteria, mycobacterial infections and particularly their treatment. Infections caused by mycobacteria are widespread worldwide and a large part of the population is infected with them each year. The most common forms are pulmonary, but practically any organ of the human body can be affected. Although these diseases are treatable with combinations of antimycobacterial drugs, the number of cases with drug resistant forms of mycobacterial diseases is currently on the rise. Therefore, emphasis is placed on the development and testing of new drugs that would be able to treat these resistant forms of diseases in the future. The experimental part is dedicated to testing newly synthesized antimycobacterial compounds that were tested using the microdilution broth method against five strains of Mycobacterium genus (M. smegmatis, M. aurum, M. avium, M. kansasii and M. tuberculosis H37Ra). The results are interpreted and evaluated in terms of the...
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Monitoring the profile of phenolic substances in different parts of apple trees by HPLC
Šilhavá, Kristýna ; Šatínský, Dalibor (advisor) ; Sklenářová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Kristýna Šilhavá Supervisor: doc. RNDr. Dalibor Šatínský, Ph.D. Consultant: Mgr. Anežka Adamcová Title of Diploma Thesis: Monitoring the profile of phenolic substances in different parts of apple trees by HPLC This diploma thesis covers the performance of a quantitative analysis of phenolic compounds in ten different cultivars in Malus. Specifically, the presence of phloridzin, phloretin, rutin, quercitrin and chlorogenic acid was monitored. Extracts of buds, flowers, leaves and bark in each species were analyzed during the four different vegetation periods. Further, the presence of phenolic compounds in samples of wood chips and fruits was monitored. The determined analytes displayed a different content profile depending on the plant material, apple cultivar and the period in which they were collected. Except for fruits, phloridzin was the dominant component in all varieties and in all materials. The highest total concentration of phenolic compounds was found in the leaves in the spring March-April 2020 in species 'Rubinstep'. This diploma thesis used a developed and validated HPLC-DAD method for a large number of extracts. A YMC-Triart C18 ExRS column (150 × 4.6 mm × 5 µm, 8 nm) was chosen...
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Monitoring the profile of phenolic substances in different parts of apple trees by HPLC
Šilhavá, Kristýna ; Šatínský, Dalibor (advisor) ; Sklenářová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Kristýna Šilhavá Supervisor: doc. RNDr. Dalibor Šatínský, Ph.D. Consultant: Mgr. Anežka Adamcová Title of Diploma Thesis: Monitoring the profile of phenolic substances in different parts of apple trees by HPLC This diploma thesis covers the performance of a quantitative analysis of phenolic compounds in ten different cultivars in Malus. Specifically, the presence of phloridzin, phloretin, rutin, quercitrin and chlorogenic acid was monitored. Extracts of buds, flowers, leaves and bark in each species were analyzed during the four different vegetation periods. Further, the presence of phenolic compounds in samples of wood chips and fruits was monitored. The determined analytes displayed a different content profile depending on the plant material, apple cultivar and the period in which they were collected. Except for fruits, phloridzin was the dominant component in all varieties and in all materials. The highest total concentration of phenolic compounds was found in the leaves in the spring March-April 2020 in species 'Rubinstep'. This diploma thesis used a developed and validated HPLC-DAD method for a large number of extracts. A YMC-Triart C18 ExRS column (150 × 4.6 mm × 5 µm, 8 nm) was chosen...
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Software for molecular dynamics
ŠILHAVÁ, Kristýna
The software for molecular dynamics provides the ability of simulating systems that consist of millions of particles, just for a few nanoseconds. This method allows for the carrying out of virtual experiments without real laboratory equipment and only with computer technology. Molecular dynamics play an important role in understanding the structure and function of biological, organic and inorganic systems. The objective of this thesis was to introduce the basics of molecular dynamics, solve model tasks in Amber software and Gromacs and to compare their capabilities. I have worked on the Hermes Computer Cluster that belongs to the Faculty of Science at the University of South Bohemia and this was part of the MetaCentrum Project. I have worked with both programs using the command line of the Linux Operating System. This thesis describes all of the file formats that are needed for working with these programs, and how they are to be used. I have built up several systems in Gromacs and Amber: 1) water 2) water and ions 3) solvated lysozyme. Based upon these sample systems, I have compared the basic utilities for preparation, the execution and the analysis of these simulations. This thesis should serve as a quick introduction and familiarization with Amber software and Gromacs.
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