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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Ensuring Security and Protecting Health during Work in the School System
 Starý, Miroslav ; Černá, Libuše (advisor) ; Mach, Václav (referee)
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Ensuring Security and Protecting Health during Work in the School System
 Starý, Miroslav ; Černá, Libuše (advisor) ; Mach, Václav (referee)
Detailed record
Immunodetection of enzymes catalyzing carbonyl reductions (Western blotting)
 Černá, Libuše ; Boušová, Iva (referee) ; Netopilová, Miloslava (advisor)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Libuše Černá Supervisor: PharmDr. Miloslava Netopilová, Ph.D. Title of diploma thesis: Immunodetection of enzymes catalyzing karbonyl reductions (Western blotting) The enzymes which reduce carbonyl group are important for metabolism of eobiotics and xenobiotics. There are recognized three superfamilies of carbonyl- reducing enzymes: aldo-keto reductases (AKR), the medium-chain dehydrogenases/reductases (MDR) and the short-chain dehydrogenases/reductases (SDR). The 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) is member of the SDR superfamily. This enzyme is essential for activating steroid hormone cortison to its active form cortisol. Its activity is also vital for metabolism of some other xenobiotics, e.g the anticancer drug oracin. Yet the carbonyl group of oracin is also reduced by another unknown enzyme wich is located in microsomal membrane. For the purification of this unknown enzyme Q Sepharose was used. The aim was to find out which fractions after purification contain 11β-HSD1 by using the imudetection (Western blotting). In Western blotting we used nitrocelulose membrane, the primary rabbit polyclonal antibodies against 11β-HSD1 dilution 1:1000, the secondary polyclonal...
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