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Mass spectrometry in lipidomics
Cvačka, Josef
Current approaches of analytical chemistry in lipidomics based on mass spectrometry and chromatography are discussed in detail. Examples of qualitative, quantitative and imaging approaches are given.
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Analysis of plant membrane lipids by RP-HPLC/HR-ESI-MS
Zábranská, Marie ; Vrkoslav, Vladimír ; Cvačka, Josef
Monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) are glycolipids forming the major part of biological membranes in photosynthesizing organisms. The individual species differ from fatty acids linked to sn-1 and sn-2 possition on glycerol backbone. Complexity of their mixtures is increased by enzymatic or chemical oxidation of unsaturated species. The aim of this work was to develop and optimize RP-HPLC/HR-ESI-MS2 methods for analysis of oxidized and non-oxidized MGDGs and DGDGs in plant samples. MGDGs and DGDGs were isolated from Arabidopsis thaliana and Melissa officinalis.
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