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Diagnostics of toxoplasmosis
Bajajová, Kateřina ; Voxová, Barbora (advisor) ; Čermáková, Zuzana (referee)
CHARLES UNIVERSITY IN PRAGUE Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Bc. Kateřina Bajajová Supervisor: PharmDr. Barbora Voxová Title of diploma thesis: Diagnostics of toxoplasmosis Worldwide widespread parasite Toxoplasma gondii (T. gondii) is an agent of toxoplasmosis infection. This infection is the most widespread antropozoona and pertain to the most frequent infectious disease, because it affects about one third of population in its latent form. For immunocompetent people infection is not a risk, its progress is asymptomatic and it causes lifelong latent infection. On the other hand for some of patients means toxoplasmosis weighty medical problem. There are mainly people with immunodeficiency after reactivation of latent form of infection, the primary infection in pregnant women, the infection is transmitted from mother to fetus and newborns with the congenital form of toxoplasmosis. The disease can has in these cases very serious consequences and fatal process. The T. gondii infection can be diagnosed by several methods. Methods are divided into direct, aimed at finding a microbe in biological material, and indirect methods, in which serologically show antibodies against infectious agens. The direct methods include histological...
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Laboratory diagnosis of hepatitis E
Vojkůvková, Pavla ; Voxová, Barbora (advisor) ; Křivčíková, Lucie (referee)
This thesis in the theoretical part contains basic information about the problems of viral hepatitis E in the world and the Czech Republic. The practical part describes the methodology used to analyze samples with suspected viral hepatitis E. The first part examined the number of analyzed samples from patients suspected of being infected with hepatitis E ( HEV ). The analysis is performed according to the positivity or negativity of the results to those years , age and gender. In the second part, patients with a confirmed diagnosis of HEV infection are divided into individual years, and analyzed by gender, month of sampling and proven class of anti - HEV . Documents provided by the analysis based on statistical data of the investigated samples of patients in the virology laboratory of the Institute of Clinical Microbiology, Faculty Hospital in Hradec Kralove in the years 2008-2011. Keywords: Hepatitis E, virus HEV, HEV ELISA, anti - HEV IgM and IgG
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Detection of Helicobacter pylori infection
Štrofová, Simona ; Voxová, Barbora (advisor) ; Drahošová, Marcela (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Bc. Simona Štrofová Supervisor: PharmDr. Barbora Voxová Title of diploma thesis: Detection of Helicobacter pylori infection Abstract The issue of Helicobacter pylori (HP) infection is a very hot topic in gastroenterology and it evolves rapidly due to a great amount of new information. HP is a spiral bacterium colonizating human gaster and duodenum. The bacterium itself is adapted to the acid enviroment of the gaster. Since its first characterization by Marshall and Warren in 1984, the HP has been known as an agens causeing gastritis, peptic ulcers and gastric cancer. Besides its well-demonstrated role in gastroduodenal disease, some authors have proposed a link between HP and number of extragastric disease involving the cardiovascular, dermatological, hematological and immunological systems. The HP infection can be diagnosed by several methods. These are classified as either invasive or non-invasive according to the use of endoscopy. The invasive ones are histological examination, rapid urease test, cultivation and some molecular techniques. The non-invasive ones are serological examination, breath test and a detection of HP antigen in stools. The last method mentioned is simple,...
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Rapid detection microogranism in pharmaceutical materials.
Novosád, Aleš ; Jílek, Petr (advisor) ; Voxová, Barbora (referee)
1 Summary Many microbiology laboratory, no matter what already in pharmaceutical firms or to hygienic stations ground and firms conversant food production used to analysis big quantity microbiology samples conventional techniques based on cultivation appropriate culture. These techniques take hours to days to yield a result, are tedious and are not suitable for non- culturable microorganisms. Further, culture-based techniques do not provide real-time information on the physiological status of the organism in situ which is important in the industrial manufacture of many microbial products (1) and often at high of the number of micro - organisms, when isn't possibility number micro - organisms determine, all time cultivation about next dilution sample elongate and about time growth this micro - organism. Flow cytometry makes it possible to obtaining real microbial determination single micro - organisms, without dependencies on type microbial culture. Nevertheless, flow cytometry has not been extensively used as a tool for routine microbial analysis. Reason is mainly high cost and complexity of instrumentation, the need for trained flow cytometrists and the lack of assay kits with appropriate biological reagents for specific applications. Much modern instruments are now relatively simply serviceable, thanks...
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