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Protein domains utilizable for development of binding molecules
Dobešová, Petra ; Malý, Petr (advisor) ; Veverka, Václav (referee)
Small protein domains represent basic building blocks of naturally occurring proteins. Many of them exhibit excellent stability, lack disulfide bonds and their structures, therefore, represent attractive tools for generation of artificial binding molecules. First step in the production of novel binding proteins is the definition of a basic domain structure, called "scaffold", which is identified using in silico approaches, resulting in discovery of mutable amino acid residues. Then, randomization of such residues leads to design of a highly complex combinatorial library as a key tool for targeted selection of protein variants. Based on chosen selection approach, the particular protein variants can be tested for their ability to recognize the target molecule with high specificity and binding affinity. Small binding proteins lack post-translation modifications, exhibit thermal stability, are resistant to many organic solvents and can be produced on a mass scale in bacterial host cells. In addition, they can be easily modified and used in vivo with excellent tissue penetration. Due to these beneficial properties, small artificial binding proteins are extraordinary useful biotechnological tools and represent a promising alternative to monoclonal antibodies. The aim of this work is to summarize our knowledge on...
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Development of the Urban Rail Transport in Prague
Jiránková, Lucie ; Zelený, Lubomír (advisor) ; Malý, Petr (referee)
This thesis is focused on the Development of the Urban Rail Transport in Prague. This thesis aims to specify the development of the Urban Rail Transport in view of the current state. In this work, a comparison is made with the suburban railway transport in Munich, the evaluation of the establishment of new stations and stops, and the forecast of the development of railway transport in Prague in the long run.
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Femtosecond laser spectroscopy of diamond
Bažíková, Sára ; Malý, Petr (advisor) ; Preclíková, Jana (referee)
Due to its extraordinary features and wide bandwidth (5.47 eV), diamond is a very promising material in the field of optoelectronics. By absorbing ultraviolet light, excited charge carriers - electrons and holes - are created in the diamond, which can create excitons due to mutual Coulomb interaction. For low temperatures and high concentrations of photoexcitated carriers, carriers can condense into electron-hole droplets and form an electron-hole liquid. The aim of this diploma thesis is to follow up with previous research at the department and to examine the dynamics of electron-hole liquid in bulk diamond at low temperatures. Using femtosecond laser spectroscopy, we investigate the influence of excitation wavelengths on the dynamics of electron-hole liquid condensation.
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Alternative ECG measurement using flexible electrodes for scanning of electrical potentials on the human body
Hylský, Václav ; Šteffl, Michal (advisor) ; Malý, Petr (referee)
The objective of this bachelor thesis is to measure ECG signal using experimental textile electrodes. The theoretical part deals with heart anatomy, the placement of the heart within the rib cage, heart's electrical activity and ways of measuring it using electrocardiography as well as alternative ECG systems. The practical part of this thesis deals with the use of an experimental ECG T-shirt and wearable electronic equipment. This part also includes the description of the examination method as well as of the equipment which processes and records the signal. The next part this thesis deals with laboratory testing of the experimental ECG T-shirt on a selected sample of people. It also includes an assessment of the measurement results. Keywords: Conductive fabric, electrocardiography, electrocardiogram, electrodes
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Ultrafast spectroscopy of hybrid nanosystems
Galář, Pavel ; Malý, Petr (advisor) ; Herynková, Kateřina (referee) ; Vrňata, Martin (referee)
Title: Ultrafast spectroscopy of hybrid nanosystems Author: RNDr. Pavel Galář Department: Department of Chemical Physics and Optics Supervisor: prof. RNDr. Petr Malý, DrSc. Abstract: This Ph. D. thesis is focused on physical phenomena located at the interface of hybrid nanostructure composed of polycrystalline diamond and polymer polypyrrole. The main method used in our experimental study was ultrafast laser spectroscopy that allowed us to gain new findings about electron recombination processes in polycrystalline diamond layers, polypyrrole and in their hybrid structures. The research was focused on mutual influence of both components, especially through energy and charge transfer. In the first step of our research we carried out optical characterisation of different kinds of polypyrrole and complex study of recombination processes dynamics of photoexcited charge carriers in polycrystalline diamond. The measurements were realized by the methods of time-resolved photoluminescence and transmission spectroscopy in the time scale from picoseconds to milliseconds. On the basis of the obtained results the model explaining the origin of luminescence signal related to the different kinds of electron recombination processes in non- diamond phase and on surface defects of diamond grains in polycrystalline layers was...
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Static analysis of C# programs
Malý, Petr ; Bednárek, David (advisor) ; Parízek, Pavel (referee)
The goal of this diploma thesis is to study and implement selected methods of static code analysis for C# programs translated into the Common Intermediate Language. The results of this work are integrated into the ParallaX Development Environment system. This diploma thesis focuses on Structural, Points-to and Dependence. analysis. Powered by TCPDF (www.tcpdf.org)
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Isometric strategy game Citadel
Pišl, Bedřich ; Brabec, Michal (advisor) ; Malý, Petr (referee)
This thesis describes design and implementation of a real-time strategy game Citadel. The emphasis is on application architecture, which uses Allegro library for graphics and controls and Boost Serialization library for saving the game. It also includes description of techniques used in implementation such as influence maps and a target system used by artificial intelligence, pathfinding algorithms, plasma fractal for map generation and a variant of Dinitz's algorithm for maximum flow. Powered by TCPDF (www.tcpdf.org)
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Information System for Conference Planning
Malý, Petr ; Zavoral, Filip (advisor) ; Čermák, Miroslav (referee)
The subject of this thesis is to design and implement an information system usable in the area of science conferences. The work introduces the reader into issue that had to be solved during the development. It will also familiarise the reader with application's structure and give the instructions for use. The program allows the user to gather and manipulate information about science conferences. The system also allows managing assignment of monetary resources and their connections with individual conferences. Therefore, it offers creation of monetary resources overviews.
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Dynamics of photoexcited charge-carriers in diamond
Popelář, Tomáš ; Malý, Petr (advisor) ; Herynková, Kateřina (referee)
Title: Dynamics of photoexcited charge carriers in diamond Author: Bc. Tomas Popelar Department: KCHFO MFF UK Supervisor: prof. RNDr. Petr Maly DrSc., KCHFO MFF UK Abstract: In this work we examine the luminescence from diamond in the strong excitation regime by one-photon or two-photon absorption of femtosecond laser beam. Measured sample was very pure type IIa diamond prepared by CVD method which was held in cryostat in order to measure temperature dependant luminescence in range from 12 K to 300 K. The signal was collected and analyzed either by spectrograph or streak camera so we gained time-integrated and time-resolved results. We analyzed only the part of the spectrum containing contributions from electron-hole liquid (for low temperatures), free excitons and probably exciton complexes. For higher temperatures where the condensation is not possible the contribution from e-h plasma was too weak compared to free excitons and was only detectable by time-resolved measurements. Other time-dependant results were obtained by pump and probe experiment with which we examined a change of life- times based on mode of excitation (one-photon or two-photon one) and also an influence of other beams on the condensation into electron-hole drops. Keywords: CVD diamond, dynamics, e-h liquid, excitons
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