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Allergic diathesis - the effect of familial burden and environment
Pagáčová, Lucie ; Jílek, Petr (advisor) ; Krčmová, Irena (referee)
Lucie Pagáčová Allergic diathesis - the effect of familial burden and environment Diploma thesis Charles University in Prague, Faculty Of Pharmacy in Hradec Králové Pharmacy INTRODUCTION: In our study we tried to demonstrate the various factors that might influence the development of allergic diseases. We focused mainly on the genetic influence on the part of parents and pointed to the influence of the siblings. The enviroment in which probands were characterized by size grew and the size of the family residence. OBJECTIVES: We tried to clarify selected factors that may cause allergic diseases. We brought in this regard the new data that compare with studies carried out abroad. METHODS: The necessary data we obtained through a questionnaire form that was designed for mothers of 12 years old children. We used 1672 questionnaires filled correctly form which we selected a group of 740 children without symptoms of allergies and we have defined a clear 626 allergic children. These two groups we compared with factors for which we have tried to demonstrate an impact on the emergence of asthma, eczema, hay fever, perennial rhinitis, hives and other symptoms. RESULTS: We haven't established the role of gender as an important factor, as well as BMI, date of birth and residence's size. We also compared the...
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Allergic diathesis - the effect of nutrition
Karbulová, Lucie ; Jílek, Petr (advisor) ; Krčmová, Irena (referee)
BACKGROUND: In recent years we could see an apparent increasing number of allergic diseases in the population. In this context, it has been described a number of protective and risk factors that affect the development of allergy. With regarding to extent of this issue we decided to address the topic of allergies in our study. We focused on issues related to maternal and child nutrition. The aim is to compare the effect of some selected factors between groups of children (allergic and non-allergic children). METHODS: We sent a total of 2635 anonymous questionnaires to schools. We focused on children aged 12 - 13 years (seven grades students). Mothers filled the questionnaires in for their children. We returned 1672 completed questionnaires back. Children were divided on allergic and non-allergic by allergic symptoms, doctor visits and potential treatment. We investigated the influence of individual factors on the development of allergies among children. RESULTS: Statistically significant as a risk factor appeared to eat honey during pregnancy and breast-feeding twice or five times a week. Among the protective factors were included exclusive breastfeeding for at least 10 - 12 months and occasional eating of a curd during pregnancy, these factors were statistically significant. However, we did not...
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Chemokines of human epithelial cells.
Eisenhamerová, Hana ; Jílek, Petr (advisor) ; Rathouská, Jana (referee)
Organism is protected by many ways. Phagocytosis belongs aminy a nonspecific defensive function. Important part of phagocytosis is called chemotaxis. It is influenced by chemokines. We know 52 different chemokines and 20 their receptors. Chemokines are divided into four groups. They differ structurally and functionally. Chemokines play a role in activation of leukocytes in inflammation, in maturitationof cells and homeostasis. Chemokines are found in various diseases.
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The use of recombinant allergens for diagnosis of allergic dieseases
Karásková, Nela ; Jílek, Petr (advisor) ; Krčmová, Irena (referee)
Laboratory diagnosis of allergic diseases is nowadays focused on the identity of specific IgE. The detection of sensitization are often used mixed allergens from natural sources with poorly defined composition. The exact specification of the allergens are tests using recombinant allergens. Recombinant allergens are not only used for the determination of specific IgE antibodies, but also to test basophiles activation. Latest test is a microarray using recombinant allergens method. In conclusion of this work are presented case reports and the results of laboratory tests that demonstrate the practical use of recombinant allergens.
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Lyme borreliosis
Kohoutová, Lucie ; Jílek, Petr (advisor) ; Křivčíková, Lucie (referee)
Author: Lucie Kohoutova Title: Lyme borreliosis (Diploma thesis) University: Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Field of study: Pharmacy Background: We investigated the presence of Lyme disease in the area of Havlickuv Brod based on laboratory diagnostics of specific or non- specific antibodies in the blood serum and in the liquor. The aim was to define typical laboratory signs of illness and recommend racional methods in diagnosis of Lyme disease. Methods: We used the data from Havlickuv Brod hospital laboratory of immunology. Patients were dividend into the groups according to the results of basic tests (if there were immunoglobulines type M and type G in serum or liquor), sex, age, the day of blood collection, code of diagnosis, patient's doctor and the type of running tests (ELISA, westernblot). These groups were compared. Patients with at least one positive result in basic tests and with any of additional test at the same time were divided in compliance with relations amongst these types of tests. We tried to determine appropriate interval between taking samples during monitoring the dynamics of the disease by contribution- based method. Results: More threatned by Lyme borreliosis are older women and people over the age of fifty- five. The highest number of new...
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Comparison of the immunophenotypes of normal and pathological plasma cells - determination of the rate of risk of progression in patients with MGUS
Kozelka, Jiří ; Jílek, Petr (advisor) ; Jankovičová, Karolína (referee)
- 7 - Abstract Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Jiří Kozelka Supervisor: PharmDr. Petr Jílek, CSc. Consultant: Mgr. Vladimíra Vroblová, Ph.D. Title of diploma thesis: Comparison of the immunophenotypes of normal and pathological plasma cells - determination of the rate of risk of progression in patients with MGUS Four-color flow cytometry is a necessary part of plasma cell imunophenotypisation today. It allows to define plasma cell count with a high accuracy and to distinguish normal and pathologic cell on the base of surface and cytoplasmatic markers expression. An expression of the following surface markers, CD138, CD38, CD19, CD56, CD45 CD20 and CD27, was monitored in this dissertation. An expression of the cytoplasmatic light chain κ and λ followed which confirmed monoclonality of the plasma cells in patients with multiple myeloma or with monoclonal gammopathy of undetermined significance. In reference group of patients the expression of light chain κ and λ confirmed polyclonality. 47 samples of patients with diagnosed multiple myeloma or with monoclonal gammopathy of undetermined significance and 22 samples from patients with increased count of plasma cell, but without present hematological disease of plasma cells,...
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