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HLA typization of celiac disease in pediatric patients
Jandová, Hana ; Řeháček, Vít (advisor) ; Jiroušová, Hana (referee)
This theses is a study of the relationship between certain HLA alleles and coeliac disease. I am concentrating on individuals with a predisposition to coeliac disease and the link between these and the presence of particular alleles. In the theoretical part of the theses I'm describing the structure of the HLA system and the association of HLA with the disease. Furthermore I am concentrating on characteristics of the disease and possibilities of its diagnoses. In the practical part I describe in detail the molecular-genetical examination of coeliac disease. I asses the presence of predisposed alleles for this disease in patients from The II. Internal Gastroenterology Clinic , The Children's Clinic of Faculty Hospital in Hradec Kralove and other gastroenterology clinics in the region.
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Cryoconservation of erythrocytes and its use in civilian and military healthcare
Sládková, Eliška ; Řeháček, Vít (advisor) ; Jiroušová, Hana (referee)
The theme of my final paper is Cryoconservation of erythrocytes and its use in civilian and military healthcare. This work consist of theoretical part and practical part. The first part of the thesis introduces cryobiology basics, types of cryoprotectants and the cryopreservation of erythrocytes itself. The thesis describes individual procedural steps leading to the final transfusion product, i.e. methods of blood collection, blood's pre- glycerolisation processing, glycerolisation itself and freezing. Furthermore, it describes issues associated with storage of frozen blood products, as well as with their thawing and deglycerolisation. The second part, which is practical, deals with quality control of cryopreserved erythrocytes. It describes various quality parameters and acquaints readers with the results of such measured parameters.
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Rh-system
Forstová, Monika ; Řeháček, Vít (advisor) ; Jiroušová, Hana (referee)
This bachelor thesis summarizes the basic information about Rh system, the second most important system of blood groups. This work is split into two parts. Theoretical part is focused on the blood - its composition and formation. Next, it focuses on history, antigens and antibodies of Rh system, prenatal and postnatal serological examination, laboratory testing of irregular antierythrocytes antibodies and hemolytic disease of a newborn. Practical part describes procedure examination screening of antibodies on the Transfusion departments in Havlíčkův Brod and detection of irregular antierythrocytes antibodies. Keywords: Rh system, antibodies, imunohematology, hemolytic disease of a newborn
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The other human red blood groups systems.
Košťálková, Helena ; Řeháček, Vít (advisor) ; Jiroušová, Hana (referee)
Immunohematology studies antigen-antibody reactions and different forms of antigens and antibodies which cause the immunologic reaction of the organism. One of the hazards of blood transfusion is the immunization of individuals. The reaction between antigens and antibodies leads to the binding of the antibodies on the red blood cells membranes and premature degradation. Development of the antibodies affects about 0.1 - 3 % of the human population. Currently 270 blood group systems are known. The National Reference Laboratory for Imunohematology determines the specificity of antibodies against the blood group systems Diego, Yt, Xg, Scianna, Dombrock, Colton, Landsteiner-Wiener, Gerbich, Cromer, Knops, Indian, Ok, John Milton Hagen, HFA and LFA. For the detection of antibodies against the mentioned blood group systems uses the laboratory proper assays and Scarf - an archive of serum with antibodies and red blood cells containing the special antigens. Over the time period since 1st January 2007 till 31st December 2011 102 antibodies against the named blood group systems were detected. Some of these antibodies are clinically significant and subsequent hemoteraphie is very complicated. The most detected antibody was anti-Wr(a), an antibody against the low frequent antigen Wr(a) Diego system.
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Good Transfusion Practice.
Sigmundová, Pavla ; Řeháček, Vít (advisor) ; Jiroušová, Hana (referee)
Autor: Pavla Sigmundová Název: Good transfusion practice Bakalářská práce Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové Studijní obor: Zdravotnická bioanalytika - zdravotní laborant (Kombinovaná forma studia) Abstract The correct practice of blood is a topic that deserves more attention. Failure to comply with the correct blood practices may affect the safe transfer of blood products. To ensure the safe transfer are therefore important theoretical knowledge of health professionals, who are entitled to the blood products. The result of the test, which I form, clearly speaks for more training in this area. The correct blood practices relating to: before transfusion procedures performed outside of the blood bank (clinical Department), before transfusion examination carried out on blood bank, handling with transfusion preparations for clinical departments. Failure to comply with best practices we will post the patient at risk of post-transfusion reactions. Even in the case of suspicion of post-transfusion reaction are important theoretical knowledge of health professionals, they should know how to proceed. In the theoretical part I summarised the correct blood practice including the indications. Is the rule, each transfusion, which is not strictly indicated, it is strictly to the continued...
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Testing of HLA antigens and antibodies in the laboratory of transfusion department.
Hájková, Lenka ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Lenka Hájková Title: Testing of HLA antigens and antibodies in the laboratory of transfusion department Bachelor's Thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Field of study: Medical Laboratory Technician Background: Retrospective study, overview of investigations of potential kidney recipients from 12 participating hemodialysis centres during 3 years (2008 - 2010). Methods: Following examinations were performed on potential kidney recipients in the HLA laboratory of the Transfusion Department, University Hospital Hradec Králové: serological fenotyping HLA-A, B (CDC test), PCR HLA-DRB1* genotyping (SSO, SSP) and screening of antileukocyte antibodies (CDC test). Results: The total number of 154 potential kidney recipients were examined during the period of time from 1. 1. 2008 to 31. 12. 2010 (2008: 57, 2009: 46, 2010: 51). Screening of antileukocyte antibodies was performed on 1 140 samples, the most frequent result of antibody levels was between 0 - 30 %. Most patiens were at age of 41 - 61 years, male/female ratio was 2:1. The most frequent HLA antigens concerning the group of patients with kidney diseases were HLA-A2, HLA-B8 and HLA-DRB1*11. Conclusions: During the period under consideration the number of newly registered potential kidney recipients did not...
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Rh - system.
Masopustová, Zdeňka ; Řeháček, Vít (advisor) ; Jiroušová, Hana (referee)
This work is focused on antigens and antibodies of the Rh system. Antibodies of the Rh system are not natural; they occur mostly as immune antibodies. Antigens of the Rh system are among the strongest immunogens with the greatest frequency. Antibody positivity was observed over a period of 5 years (2005 - 2009). Approximately 3500 blood samples per year were examined in screening tests and approximately 15 kinds of different antibodies with different occurrence frequency were identified. In some cases identification required more complicated procedures. The foundation of this work was acquired at the Transfusion and Haematology Ward at Kroměříž Hospital.
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