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Ovlivňuje obsah buněčných mastných kyselin a enzymů jeskynních bakterií potravní preferenci .i.Enchytraeus crypticus./i. (Oligochaeta, Enchytraeidae)?
Krištůfek, Václav ; Elhottová, Dana ; Šustr, Vladimír ; Lasák, R. ; Kováč, L. ; Mock, A. ; Luptáčik, P.
Cellular fatty acid screening (MIDI System) of 93 bacterial strains isolated from the Domica Cave in the Slovak Karst region ( Slovakia) showed that three bacterial strains (.i.Chryseobacterium./i. sp., .i.Enterobacter amnigenus, Rhodococcus./i. sp.) produce polyunsaturated fatty acids (PUFA) 18:3w6 and 20:4w6. These species (along with a non PUFA producer, .i.Rhizobium./i. sp.) were isolated from the gut content or body surface of .i.Mesoniscus graniger./i. (Frivaldsky, 1865) (Crustacea: Isopoda). Bacterial strains were tested for activity of nine saccharolytic enzymes. .i.Chryseobacterium./i. sp. showed amylase, maltase and cellobiase activity, other bacterial species only had amylase activity. As PUFA and enzymes may be essential for animal growth and development, colonies of the four strains were grown for further use in laboratory food selection and reproduction experiments with .i.E. crypticus./i..
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Použití metody Sherlock MIS System pro identifikaci bakteriálních izolátů z lesního opadu – předběžné výsledky
Jirout, Jiří ; Petrásek, Jiří ; Elhottová, Dana ; Krištůfek, Václav ; Nováková, Alena ; Rusek, Josef
This work has been related to the effect of climatic changes (especially warming) on communities of forest litter bacteria. We simulated this effect with use of litter bags with beech and/or oak litter transported to beech and spruce forest. After 2, 6 and 12 months of exposition we collected and analysed the samples. We measured quantitative and qualitative characteristics of bacteria and micromycetes community structure in all variants of forest/litter combinations (number of CFU, description of bacterial morphotypes, identification of dominant bacterial morphotypes with MIDI Sherlock Microbial Identification System). We found out that there were differencies in diversity in all variants in exposition time. From the first to the third sampling the CFU bacterial number has decreased whereas CFU micromycetes number has increased. Multivariate comparison (PCA based on relative distribution of bacterial morphotypes in samples) separated the oak litter bacterial community in spruce forest soil as the most different variant among the others.
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Vliv půdní fauny na mikrobiální společenstvo v místech po těžbě hnědého uhlí - předběžná data z mikrokosmového pokusu
Frouz, Jan ; Elhottová, Dana
The effect of several guilds of soil fauna (mesofauna, litter feeding macroarthropods, litter dwelling earthworms and epigeic earthworm Lumbricus rubellus) and two litter types (Almus glutinosa or Salix caprea) on microbial community was studied in laboratory microcosms using phospholipid fatty acids (PLFA) approach. Increasing input of organic matter in to mineral soil madiated by fauna activity result in increase of total PLFA. Some groups of PLFA such as terminally branched saturates and straight monoenoics reflected this gradient by increasing its proportion in community, opposite was true for branched monoenoics and straight poyenoics
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Růstová charakteristika a skladba funkčních skupin půdního mikrobiálního společenstva
Černá, B. ; Elhottová, Dana ; Šantrůčková, Hana
Oxyggen consumption was used for measuring growth parameters of functional microbial groups in two soils of different texture after addition of mineral, sugar-ammonium and complex media. Phospholipid fatty acids analysis (PLFA) was used to determine differences in community composition and structure. Growth parameters and composition based on a complete PLFA pattern of the microbial community, changed as a response to medium addition and type of incubation. The proportion of actinomycetes decreased whereas the proportion of fungi increased after addition both of the sugar-ammonium and complex media. Gram-negative bacteria dominated after addition of the substrates rich carbon source, while Gram-positive bacteria prevailed after addition of the carbon and nitrogen rich medium.
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