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Aspects of an experimental solution with the use of geothermal energy in Balneotherapy
Miček, Denis ; Horák, Petr (referee) ; Kabele, Karel (referee) ; Hirš, Jiří (advisor)
The Earth, our home planet is world unlike any other. The third planet from the sun is the only place in the known universe confirmed to host life. We, the humans always headed up to reach set up goals, innovate our technologies and way of living. The fact that, we had never been aware of is that this race to our bright and modern future is destroying our home. By this time, the global temperature arises by 1,9 oC since 1880, 427 gigatons of ice sheets were melted and there are 414 ppm of carbon dioxide particles in our atmosphere. As the trend is changing and we are above to think in green way of living, there is necessity to set up roadmaps and actions plans to stop, eventually decrease the damage which was already caused. The European union started to measure and provide actions plans to decrease the pollution and production of greenhouse gases since the year 1990 – which was set up as reference year for comparison to progress. This arrangement forced the countries to start evaluating the current statuses of their public and industrial sectors in terms of climate changes and provide climate plans for reducing the greenhouse gases. The most important part of climate action plans is to involve the renewable sources of energy into all public and industrial sectors and in this way start the green way of living. Therefore, the investigation and proper understanding of this problems must be the main focus of technical universities and scientific sectors. This dissertation thesis is the summit of 8 years studies and investigation in field of renewable sources of energy, mainly focused on geothermal energy which production in year 2002 was 0 GWh. The main goal is to experimentally analyze designed solutions, elaborated within master and bachelor thesis in real operation of Balneotherapy departments at Slovak Health Spa Piestany, Slovakia from energy, economic and environmental point of view, to set up energy, aqua management and fully understand the way of involvin
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Heat Pump in the Heating System
Andrš, Matěj ; Počinková, Marcela (referee) ; Horák, Petr (advisor)
The bachelor thesis deals with the design of heating of a newly built family house. The theoretical part introduces heat pumps and deals with their basic characteristics and types of heat pumps. The calculation part describes the specific design of the heating of the building and the design of individual components in the heating system. A ground/water heat pump is proposed as the heat source, where heat will be extracted from the ground using deep boreholes. The heating of the building will be done by underfloor heating. The hot water will be prepared in a heat pump. The technical report then describes the design and technical requirements.
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Building Energy Performance Certificate
Farkhutdinova, Iuliia ; Horká, Lucie (referee) ; Horák, Petr (advisor)
In the bachelor thesis, the author focuses on the evaluation of an apartment building's energy efficiency in Ostrava before and after its reconstruction. In the theoretical part (A), the basic concepts of energy performance of buildings and the relationships between them are presented. In the calculation part (B), the comparison of the energy needs and an energy evaluation of the building before and after its reconstruction is provided. The assessment is performed by an energy performance certificate (PENB). Its part is the proposal of cost-saving measures for the current state of the building after reconstruction. The results consist of the three energy performance certificates presented in the part C.
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Energy Optimisation of the Building
Kozla, Ján ; Vyhlídalová, Karolína (referee) ; Horák, Petr (advisor)
The bachelor’s thesis deals with energy assessment and optimalization of the polyfunc-tional building. The theoretical part focus on the topic of solar electric generation, using photovoltaic panels and solar thermal plants. Calculation part shall analyse the energet-ical flow of the building, based on thermal properties of the building envelope and tech-nical amenities. Furthermore, I propose austerity measures to optimization energy inten-sity, witch I analyse form an energy and economic point of view at the end of the calcula-tion part. Part C – The PENB project contains energy performance certificate of the initial state and the optimised state.
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Design of a renewable heat source using an energy performance certificate
Karas, Richard ; Vyhlídalová, Karolína (referee) ; Horák, Petr (advisor)
The bachelor's thesis deals with the design of a renewable heat source of the existing kindergarten building in Janovice. The design is made using the building's energy performance certificate. The theoretical part deals with renewable energy sources for heating, which specifically focuses on thermal energy and biomass. The calculation part deals with the energy analysis of energy needs and flows of the building, the energy needs for individual building systems and in coclusion the economic and ecological evaluation of design states. The project part contains three energy performance certificates of assessed building.
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Diversity of methods used for characterization of molluscan hemocytes
Jindrová, Zuzana ; Horák, Petr (advisor) ; Skála, Vladimír (referee)
Hemocytes are the main immune cells of invertebrates; therefore they can be found in molluscs, too. They differ both in morphology and function. The two generally accep- ted morphological types, granulocytes and hyalinocytes, vary in the level of phagocy- tosis and encapsulation, production of reactive oxygen species and nitrogen oxide, and presence of some enzymes. There is an array of methods by means of which hemocy- tes can be characterized. Microscopy serves particularly for study of morphology. An- tigens localized on the surface can be determined by monoclonal antibodies or lectin probes. Hemocytes can be divided on the basis of cell size and granularity using gra- dient centrifugation or flow cytometry. Production of nitrogen oxide and reactive oxy- gen species is monitored by adding appropriate substrate which changes its proper- ties after reaction with the radical. It may become fluorescent, change absorbance of the solution or form a visible precipitate. Another possibility is the use of chemilu- miniscence. The objective of hemocyte research is to explain mollusc-pathogen inter- action. 1
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Effect of selected antiparasitic drugs on Trichobilharzia regenti
Lípová, Pavlína ; Horák, Petr (advisor) ; Chanová, Marta (referee)
Praziquantel is a drug of choice for the treatment of schistosomiasis caused by human species of the genus Schistosoma. The effect of the drug on avian schistosomes is not completely known. Monensin is a drug used for the treatment of coccidiosis, and its effect on avian schistosomes is unknown. In the thesis, we studied the effect of these drugs on avian schistosome Trichobilharzia regenti. Under in vitro conditions, both drugs were effective after 6 hours of incubation. Praziquantel causes an extensive vacuolization of the whole body, while monensis treatment causes vacuolization of the anterior part of body. Higher concentration of praziquantel causes depolymerization of myofilaments and separation of surface membranes. Monensin did not impair muscles, but caused separation of tegumental layer from the basal membrane. Schistosomula in ducks were not killed by the drugs under in vivo conditions, but the worms were damaged by the drugs. Praziquantel caused vacuolization of the tegument and separation of membranes from the surface. Only vacuolization of the tegument occurred in schistosomula from ducks treated with monensin . No damage to the muscles was observed in the case of both drugs. Histological evaluation of the nerve tissue showed that thero no difference between treated and control duck....
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Molecular and serologic diagnosis of infections caused by Trichobilharzia
Vaščiková, Michaela ; Horák, Petr (advisor) ; Ditrich, Oleg (referee)
Cercariae of the genus Trichobilharzia can penetrate not only the skin of definitive hosts (ducks), but they are also able to penetrate the skin of accidental hosts (mammals). As a result of the penetration, the inflammatory response known as cercarial dermatitis appears. The goal of our thesis is to detect parasite DNA in the serum and cerebrospinal fluid of infected ducks, and also in the serum of infected mice. By using PCR with primers designed for a tandem repeated sequence, we were able to detect 1 femtograms of parasite DNA isolated from sera of infected ducks. We were able to amplify parasite DNA only from 16 samples of sera and cerebrospinal fluid of the infected ducks, but we were not able to do so with the serum of mice. Sera of infected mice were also tested by ELISA and Western blot. The homogenates of T. regenti (TRhc), T. szidati (TShc) and S. mansoni (SMhc) cercariae were selected as an antigen. The results showed progressive increase in the level of IgM antibody from 10 days after 1st infection and also increase of the level of IgG from the 2nd infection. 10 days after the 4th infection, the level of IgM and IgG gradually declined, but the level of antibodies 100 days after the 4th infection was still higher if compared to uninfected mice. Results from Western blot analysis...
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The role of humoral factors in the snail immune response against schistosomes
Košťáková, Monika ; Horák, Petr (advisor) ; Dvořák, Jiří (referee)
Digenetic trematodes such as Schistosoma mansoni use molluscs, mainly Gastropoda in their life cycle, as their intermediary hosts. e internal defense system (IDS) of snails is composed of immune cells called hemocytes, which are the main effectors and act jointly with soluble components. Humoral factors could in uence directly the parasite's larval stage, the activity of hemocytes and also may serve in recognition of the parasite. Lectins are considered to be the main component of humoral immunity. ey have a primary role in non-self recognition. Other protein group with lectin-like activity called FRePs was found in Biomphalaria glabrata. eir unique structure contains a brinogen and an immunoglobulin-like domain. Cytokine-like molecules may play very important role in defense as well. Many molecules are present in hemolymph and their levels change during infection. e response to parasitosis is therefore very complex and still awaits further clari cation.
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Effects of microbiota on defense system of earthworms
Dvořák, Jiří ; Bilej, Martin (advisor) ; Kopečný, Jan (referee) ; Horák, Petr (referee)
Earthworms are important soil invertebrate organisms that participate in nutrient cycling in terrestrial ecosystems and in the formation of the soil profile from the physical, chemical and also microbial point of view. Soils are considered the most microbially diverse environments on earth. All invertebrates living in soil therefore need to possess a complex immune system. Earthworms are used as a model organism in immunology for decades. Their simple body plan consists of two main body cavities: true coelom and digestive tube. Both coelomic cavity and digestive tract represent open systems with permanent contact with soil microorganisms. Eisenia andrei species is used as a standard immunological model in our laboratory for many years. E andrei earthworms live in compost, microbially abundant environment, which is reflected in their well-developed immune system. Some new mechanisms of E. andrei defense system are described in this work. Two novel pattern recognition receptors (PRRs), Toll-like receptor (TLR) and lipopolysaccharide binding protein/bactericidal permeability-increasing protein (LBP/BPI) were characterized in earthworms. These molecules are expressed in coelomocytes and their production is upregulated after microbial challenge. Moreover, both receptors were detected in digestive tract...
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