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Individual ....
Tomeček, David ; Kocna, Petr (advisor) ; Průša, Richard (referee)
13 C-MTG breath tests are used for exocrine pancreas diagnostics. It is a quite expansive test and its usage has never been tested in clinical practice in long time horizon. This study is engaged in pre-analytical phase and contains a group of 4 people who were tested for 6 months and this is the first study ever that has been proceeded in a long time horizon. The half-year profile of these 4 people showed that some of those results are spread of tens percents against its average. The test of CO2 stability showed that the value of CO2 is independent on the temperature which the breath bag is stored in. Its concentration was same for the time of 7 days. Now we can say that the breath bags are insensitive in case of transporting and storage. The question is, why the value of delta 13 C/12 C changed. The results of this study point on fact that values of cumulative dosage recovery of these 4 people did change.
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Immunoflulorescent detection of endomysial antibodies (EmA) in the coeliac diagnosis
Martikán, Petr ; Kocna, Petr (advisor) ; Průša, Richard (referee)
The aim of this thesis was comparing of a several immunofluorescence methods and substrates for detection endomysial antibodies (EmA). The common method (Anti-Endomysium antibodies AEA) is used by UKLBD in VFN and by the some samples gives ambigious results owing to another autoantibodies (e.g. ANA, AMA, ASMA). The common method was compared with another sets (IFA-EmA-IgA - AEMAk 03a-10, ImmuGlo anti Endomysial Antipody EMA IFA Kit). Another aim of the work was how to work with this method and be able to evaluate samples by the fluorescence microscope. (...) This thesis brought me a lot of valuable experiences in laboratory and also teached me how to evaluate endomysium antibodies by indirect immunofluorescence method.
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