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Proposal of Marketing Support of Newly Introduced Products
Horáček, Pavel ; Horáček, Pavel (referee) ; Zich, Robert (advisor)
A company engaged in dog food production has recently finished the development of new unique and exceptional products. The Bachelor's Thesis tries to suggest an effective marketing support for successful introducing of these products to the market. The focal methods used by treating this thesis are especially marketing mix and other marketing methods. The outputs of the thesis are concrete proposals of marketing support of newly introduced products and the way of communication with the consumers.
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Production of extracellular polymeric substances by Aureobasidium pullulans
Horáček, Pavel ; Breierová, Emília (referee) ; Márová, Ivana (advisor)
The diploma thesis is focused on the study of the influence of cultivation conditions and arrangement for the production of extracellular polymeric substances by using yeast-like microorganism Aureobasidium pullulans. In the theoretical part a brief description of A. pullulans, its use in biotechnology and produced exobiopolymers, especially pullulan and poly-L-malic acid are presented. The first aim of the experimental part was to set the most appropriate cultivation conditions for A. pullulans CCM 8182. Growth and production properties in optimum conditions were compared with cultivation on waste substrates - oat bran, buckwheat husks, apple fiber and others. Waste substrates can be used as cheap nutrient sources which enable reducing cost of potential biotechnological production. As a further part of this work, optimization of HPLC/RI method for analysis of exobiopolymers has been done. Optimal mobile phase composition and chromatography conditions were proposed. Column Roa organic acid H+ was the most suitable for simultaneous separartion of glucose and malic acid. Before HPLC analysis hydrolysis of polymers was done. Sulphuric acid (5 mmol/L) was used as a mobile phase at flow rate 0.5 mL/min and temperature 60 °C. The highest production of pullulan occurred using oat bran as a substarate (13.03 g/L) at an initial pH 7.5. Maximum production of poly-L-malic acid was observed during the cultivation on apple peels (2.89 g/L) at pH 6. It was found that the higher production of poly-L-malic acid occurred at pH 6, while higher production of pullulan was at pH 7.5.
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Antibacterial gels and their effectivity
Horáček, Pavel ; Vítová, Eva (referee) ; Vránová, Dana (advisor)
The Bachelor thesis focuses on the evaluation of the effectiveness of disinfectants and antibacterial hand gels, which are a good complement to personal hygiene and other factors to prevent various diseases, transmitted through the hands. A brief description of skin structure, classification of microflora on the hands, including a list of potential pathogenic microorganisms located on human skin and causing many diseases can be found in the theoretical part of this work. Content of the experimental part of this work was determination the effectiveness of tested antibacterial agents by indirect determination of the number of microorganisms on naturally contaminated hands by the cultivation method. Experimental part of this work evaluates the characteristics of the tested products by potential users in the form of sociological research.
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