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Utilization of pulse labelling techniques for studying the dynamics of proteins and protein complexes
Polák, Marek ; Novák, Petr (advisor) ; Obšil, Tomáš (referee) ; Vrbacký, Marek (referee)
(In English) Mass spectrometry (MS) techniques are routinely used to probe the structure and dynamics of proteins and protein complexes. Although MS techniques lack the high resolution of data provided by X-ray crystallography, NMR, or cryo-EM, they excel in providing insights into analyte dynamics, structure, and interactions with other components, such as ligands. This doctoral thesis presents a contribution to the field of structural biology employing and extending covalent labelling approaches, namely Fast Photochemical oxidation of Proteins (FPOP) and oxidation by singlet oxygen (1 O2). These approaches were followed to study the structure, dynamics, and interaction of proteins, nucleic acids, and protein-DNA complexes in solution. Initially, FPOP was used to investigate the interaction interface of FOXO4 and DAF16-DNA response element and to show the possibilities of analyzing such a complex using both 'bottom- up' and 'top-down' approaches. Furthermore, an isotope depletion strategy combined with multiCASI-ECD proved effective in delivering structural information with the highest possible resolution for mapping protein-DNA interfaces. This research showcases how information derived from structural proteomic methods can guide the construction of in-silico models for protein-DNA complexes with...
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Comparison of Possibilities of Real Estate Financial Assurance
Erberová, Lenka ; Polák, Martin (referee) ; Vítková, Eva (advisor)
Subject of master thesis “Comparison of the possibilities of financial security of real estate” is the issue of alternative funding of housing in Czech republic. This thesis deals with the major housing finances products. It surveys building society accounts and mortgage loans withing the selected financial institutions. In the practical part these products are compared in an effort to determine the best form of securing of chosen property whose value was determined by estimation of cost of the property for credit management.
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Behaviour-Based Identification of Network Devices
Polák, Michael Adam ; Holkovič, Martin (referee) ; Polčák, Libor (advisor)
Táto práca sa zaoberá problematikou identifikácie sieťových zariadení na základe ich chovania v sieti. S neustále sa zvyšujúcim počtom zariadení na sieti je neustále dôležitejšia schopnosť identifikovať zariadenia z bezpečnostných dôvodov. Táto práca ďalej pojednáva o základoch počítačových sietí a metódach, ktoré boli využívané v minulosti na identifikáciu sieťových zariadení. Následne sú popísané algoritmy využívané v strojovom učení a taktiež sú popísané ich výhody i nevýhody. Nakoniec, táto práca otestuje dva tradičné algorithmy strojového učenia a navrhuje dva nové prístupy na identifikáciu sieťových zariadení. Výsledný navrhovaný algoritmus v tejto práci dosahuje 89% presnosť identifikácii sieťových zariadení na reálnej dátovej sade s viac ako 10000 zariadeniami.
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Risk analysis of investment structural projects
Motyčková, Denisa ; Polák, Martin (referee) ; Korytárová, Jana (advisor)
The thesis contains risk analysis of the investment construction projects. The theoretical part is focused on risk definition, types of risks and risk management methods. Further there is various risk analysis in investment projects described. In the practical part is the evaluation of economic efficiency including the risk assessment in development project of the company Prologis.
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Tax Advising
Psohlavec, Karel ; Hochman, Karel (referee) ; Polák, Michal (advisor)
Bachelor thesis examines the circulation of documents in the selected firm and its archiving in conjunction with legislative constraints and possibilities. It deals with the issue of effectiveness of this system. It compares the document obligations of three parts of the company and their mutual conection and proposes more efective ways of dealing with this system using the internal guidelines.
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Optimization of Tax Payment by Company Proprietors – Natural Persons
Hamplová, Lucie ; Škrabiš, Václav (referee) ; Polák, Michal (advisor)
This bachelor thesis is concerned with optimization of tax payment by company proprietors, executive heads of limited liability companies. It results from the rules of business law and labor code. The applied part includes an analysis of current financial situation of a company and models for assigning optimal tax payments by company proprietors, executive heads. At the conclusion, the bachelor thesis evaluates which model is optimal for the company proprietor and the company.
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Tax Optimalization of the Selected Juridical Subject
Měcháčková, Zuzana ; ,, Zuzana Křížová (referee) ; Polák, Michal (advisor)
Destination bachelory project is proposed methods as optimalization tax responsibilities by juridical subject engage agriculture activities in the period 2009 - 2011, explain the attitude tax strain with the regard for the transformations in the tax laws, introduce the occassions as tax responsibility reduce and to the conclusion present the most convenient method of the law.
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Travel Reimbursement
Maršalová, Pavlína ; Weissová, Helena (referee) ; Polák, Michal (advisor)
Bachelor’s work deals with problems travel reimbursements. Summarizes theoretical knowledge and looks for optimal rules and procedures of providing travel reimbursements in the company. The practical part contains analysis of present circumstances in the company and concept of giudeline for the travel reimbursements. In conclusion deals with examples of travel reimbursements in company and demonstrates possibility of using the guideline.
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