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Evaluation of the properties of new types of zwitterionic stationary phases in HILIC
Nováková, Daniela ; Nováková, Lucie (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Daniela Nováková Supervisor: prof. PharmDr. Lucie Nováková, Ph.D. Title of the diploma thesis: Evaluation of properties of new types of zwitterionic stationary phases in HILIC The aim of this thesis was to compare six new zwitterionic Astra ZWIX columns (3 μm, 2,1 x 100 mm) from Chromservis s.r.o. Five groups of compounds were used for the analysis, namely nucleic bases and nucleosides, nicotinic acid derivates, hydrophilic vitamins, beta- blockers, and phenolic acids. These groups of compounds were also analysed on commercially available columns - BEH Z HILIC, SYNCRONIS, OMEGA SUGAR, BEH HILIC and BEH AMIDE (OMEGA SUGAR 3 μm, 2,1 x 100 mm; other columns 1,7 μm, 2,1 x 100 mm). The investigated parameters were retention time, peak resolution, peak symmetry, and peak width at half height. Optimisation to improve peak separation of nucleic bases and nucleosides, hydrophilic vitamins, and beta-blockers was also carried out. The mobile phase gradient was optimized, as well as the column temperature, and mobile phase flow rate. The selectivity of the columns was found not to differ within the batch. The retention time repeatability condition was met for all analytes on all columns, except for...
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