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6S RNA as a tool for increased production of clinically relevant antibiotics
Kostílek, Štěpán ; Bobek, Jan (advisor) ; Ulrych, Aleš (referee)
Streptomyces belong to the group of soil microorganisms, and they are significant producers of biologically active substances used in a wide range of industries, whether it is medicine, agriculture or biotechnology. It is for this reason that the research of these microorganisms is of interest to a number of research institutions. One of the most important groups of these biologically active substances are antibiotics. A significant part of antibiotics produced in nature is produced precisely by streptomyces, and therefore it is in our interest to understand the mechanisms that influence this production. A wide variety of different regulatory mechanisms controlling antibiotic production have been found so far in streptomyces. One of these regulatory mechanisms is the utilization of scr3559 RNA. Scr3559 RNA is a small, non-coding RNA molecule that regulates antibiotic production at the level of DNA transcription. These small RNA molecules mimic the structure of the target promoter sequence and are able to interact with RNA polymerase. The presence of scr3559 RNA has not been confirmed in streptomyces as of yet, but new scr3559 RNA molecules are still being sought. To find new molecules, in silico methods are used, where the similarity of the scr3559 RNA sequence of S. coelicolor with homologous...
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