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National Repository of Grey Literature

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Immunophenotyping of mature B-cell neoplasms Babuňková, Eva ; Jílek, Petr (advisor) ; Smolej, Lukáš (referee) Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Mgr. Eva Babuňková Supervisor: PharmDr. Petr Jílek, CSc. Title of rigorous thesis: Immunophenotyping of mature B-cell neoplasms This thesis focuses on the B-lymphoproliferative diseases immunophenotype assessed by flow cytometry, which is a method currently used for the diagnosis and classification of these diseases. Apart from the diagnostic value, flow cytometry can be used for minimal residual disease monitoring after the treatment of hematological malignancies (e.g. chronic lymphocytic leukemia). A great advantage of flow cytometry is the possibility of analysing virtually any biological material. In our work we investigated peripheral blood and bone marrow. The aim of the study was to compare the expression of selected surface markers in patients suffering from different B- lymphoproliferative disorders in peripheral blood and bone marrow. We analysed 171 samples (79 peripheral blood samples and 92 bone marrow samples), which were measured on a flow cytometer BD FACS Canto II. Seventy-five women and ninety-six men we included in the study. Peripheral blood samples and bone marrow samples were sorted into the following groups: CLL, MCL, B-NHL NOS and other B... Detailed record

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