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The repair of the oxidative DNA damage in Chinese hamster cells deficient in nucleotide excision DNA repair.
 Strejčková, Lada ; Hochmann, Jiří (advisor) ; Polívková, Zdeňka (referee)
Nucleotide excision repair (NER) is one of the mechanisms of how to repair DNA damaged by the external influences. First of all, it plays the role when removing the results caused by UV radiation. The damaged bases in the form of pyrimidine dimers are enzymatically splitted out from DNA as part of oligonucleotides. The effect of the UV light on DNA is however manifested also indirectly, by oxidation of the bases. In our experiment we tried to find out whether the cells deficient in nucleotide excision repair are able to repair the damages induced only by oxidative agent. We stated the extent of DNA damages in the cells in vitro and the ability of their re- paration after treatment with the dilution of hydrogen peroxide in different concentrations. During the experiments we used the cell lines of a Chinese hamster, namely the parental cell line AA8 and their derivative UV-20, which is defective in gene ERCC1. This gene takes part in creation of nucleases participating in NER. For measuring of DNA lesions we used the method of alkaline comet assay, the ba- sis of which is alkaline single-cell gel electrophoresis. The principle of this method consists in the detection of DNA strand breaks originating in the damaged alkali-labile sites. We evaluated the number of DNA single-strand breaks. We detected...
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