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The effect of air pollution on oxidative stress markers in newborns
Ambrož, Antonín ; Rössner, Pavel (advisor) ; Rubeš, Jiří (referee) ; Gábelová, Alena (referee)
In everyday life, humans are exposed to toxic substances of anthropogenic origin. These substances can also be found in the ambient air and their impact poses a long-term risk for human health. Respirable particulate matter (PM) of aerodynamic diameter < 2.5 µm (PM2.5) is intensively studied, along with carcinogenic polycyclic aromatic hydrocarbons (PAHs), bound to it, such as benzo[a]pyrene (B[a]P), a reference carcinogenic PAH. Owing to small size, PM2.5 can penetrate the human body primarily via the airways and represent an increased health risk compared to larger particles. The negative health impacts of anthropogenic PM2.5, generated e.g. by fossil fuel combustion, are linked with its small size, relatively large surface, as well as with PAHs and other substances adsorbed on PM surface. PAHs, generated by an incomplete combustion of organic matter, can enter organism either via ingestion of contaminated food, water or via inhalation of polluted air. PAHs affect organisms via genotoxic, mutagenic, carcinogenic, embryotoxic and other adverse effects. One of the common denominators of these effects is oxidative stress, which is also considered to be the main mechanism of action caused by PM in the human organism. Oxidative damage induced by reactive oxygen species (ROS) may affect any cellular...
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The effect of maternal nutrition on the newborn genome methylation
Pavlíková, Jitka ; Rössner, Pavel (advisor) ; Černá, Marie (referee) ; Hlaváč, Viktor (referee)
The maternal diet during pregnancy is an important factor involved in the epigenetic programming of the offspring. In addition to the nutritional aspect, the epigenome is also influenced by possible contaminating substances that may occur in the diet of pregnant women. An important group of these potential contaminants are persistent organic pollutants, which are compounds of mostly anthropogenic origin that persist in the environment for a long time, become part of food chains, and some of them accumulate in living organisms. These substances are being monitored for their adverse effects on organisms, and the use of some of them has already been banned or at least significantly restricted. The aim of this work was to find out whether the quality of the diet of pregnant women in the Czech Republic differs in terms of nutrition and contamination with persistent organic pollutants so much that these differences can be reflected in the methylome of newborns. The diet quality of 49 pregnant women from the Karviná and České Budějovice regions was analyzed. During the last month of pregnancy, these women recorded in detail all the food they ate for one week and at the same time collected a quarter of this food in boxes (one box for one day). Information on the quantity and quality of the diet was...
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The use of "omics" methods in molecular-epidemiologic study in newborns from different localities of the Czech Republic
Hoňková, Kateřina ; Rössner, Pavel (advisor) ; Gábelová, Alena (referee) ; Bláha, Luděk (referee)
The "omics" is a concept of biological disciplines that globally characterizes and quantifies biomolecules involved in the key functions of an organism. The "omics" methods are used e.g. in molecular epidemiology, where they help to evaluate potential biomarkers that identify the impact of environmental factors for human health. In this thesis, the "omics" methods were applied in samples collected from newborns born in localities of the Czech Republic mostly differing by pollution levels from industrial sources. The principal aim was to determine whether environmental changes during prenatal development can affect gene expression and its regulation in newborns. The thesis further aimed to evaluate the level of air pollution at the time of biological samples collection. Using the whole genome approach, differentially expressed genes (DEGs) in newborns from districts Karvina and Ceske Budejovice (CB) were identified. In a pilot study of a small group of newborns from districts Most and CB, differentially methylated CpG sites in DNA were assessed. These sites attenuate gene activity and could be responsible for long-term changes at the genetic level. Finally, the aim was to find differentially expressed small non-coding RNA (DE miRNA) in newborns from Most and CB. Samples of umbilical cord blood from...
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The effect of air pollution on oxidative stress markers in newborns
Ambrož, Antonín
In everyday life, humans are exposed to toxic substances of anthropogenic origin. These substances can also be found in the ambient air and their impact poses a long-term risk for human health. Respirable particulate matter (PM) of aerodynamic diameter < 2.5 µm (PM2.5) is intensively studied, along with carcinogenic polycyclic aromatic hydrocarbons (PAHs), bound to it, such as benzo[a]pyrene (B[a]P), a reference carcinogenic PAH. Owing to small size, PM2.5 can penetrate the human body primarily via the airways and represent an increased health risk compared to larger particles. The negative health impacts of anthropogenic PM2.5, generated e.g. by fossil fuel combustion, are linked with its small size, relatively large surface, as well as with PAHs and other substances adsorbed on PM surface. PAHs, generated by an incomplete combustion of organic matter, can enter organism either via ingestion of contaminated food, water or via inhalation of polluted air. PAHs affect organisms via genotoxic, mutagenic, carcinogenic, embryotoxic and other adverse effects. One of the common denominators of these effects is oxidative stress, which is also considered to be the main mechanism of action caused by PM in the human organism. Oxidative damage induced by reactive oxygen species (ROS) may affect any cellular...
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The effect of air pollution on oxidative stress markers in newborns
Ambrož, Antonín
In everyday life, humans are exposed to toxic substances of anthropogenic origin. These substances can also be found in the ambient air and their impact poses a long-term risk for human health. Respirable particulate matter (PM) of aerodynamic diameter < 2.5 µm (PM2.5) is intensively studied, along with carcinogenic polycyclic aromatic hydrocarbons (PAHs), bound to it, such as benzo[a]pyrene (B[a]P), a reference carcinogenic PAH. Owing to small size, PM2.5 can penetrate the human body primarily via the airways and represent an increased health risk compared to larger particles. The negative health impacts of anthropogenic PM2.5, generated e.g. by fossil fuel combustion, are linked with its small size, relatively large surface, as well as with PAHs and other substances adsorbed on PM surface. PAHs, generated by an incomplete combustion of organic matter, can enter organism either via ingestion of contaminated food, water or via inhalation of polluted air. PAHs affect organisms via genotoxic, mutagenic, carcinogenic, embryotoxic and other adverse effects. One of the common denominators of these effects is oxidative stress, which is also considered to be the main mechanism of action caused by PM in the human organism. Oxidative damage induced by reactive oxygen species (ROS) may affect any cellular...
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The effect of air pollution on oxidative stress markers in newborns
Ambrož, Antonín ; Rössner, Pavel (advisor) ; Rubeš, Jiří (referee) ; Gábelová, Alena (referee)
In everyday life, humans are exposed to toxic substances of anthropogenic origin. These substances can also be found in the ambient air and their impact poses a long-term risk for human health. Respirable particulate matter (PM) of aerodynamic diameter < 2.5 µm (PM2.5) is intensively studied, along with carcinogenic polycyclic aromatic hydrocarbons (PAHs), bound to it, such as benzo[a]pyrene (B[a]P), a reference carcinogenic PAH. Owing to small size, PM2.5 can penetrate the human body primarily via the airways and represent an increased health risk compared to larger particles. The negative health impacts of anthropogenic PM2.5, generated e.g. by fossil fuel combustion, are linked with its small size, relatively large surface, as well as with PAHs and other substances adsorbed on PM surface. PAHs, generated by an incomplete combustion of organic matter, can enter organism either via ingestion of contaminated food, water or via inhalation of polluted air. PAHs affect organisms via genotoxic, mutagenic, carcinogenic, embryotoxic and other adverse effects. One of the common denominators of these effects is oxidative stress, which is also considered to be the main mechanism of action caused by PM in the human organism. Oxidative damage induced by reactive oxygen species (ROS) may affect any cellular...
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How trees respond to air pollution in the city
TONCAROVÁ, Gabriela
The aim of this thesis was to determine the response of trees to air pollution in the city. For this purpose, APTI index (Air pollution tolerance index), the measurement of chlorophyll fluorescence and the analysis of the content of photosynthetic pigments were used. The examined trees were Platanus acerifolia and Prunus subhirtella v. autumnalis rosea growing in Lannova třída in České Budějovice. For evaluation of APTI index, four parameters are required. These are the quantity of ascorbic acid and the photosynthetic pigments in the leaves, the relative water content in the leaves and the leaf extract pH. The samples of leaves were taken in August 2015. From Lannova třída, three trees of each species were chosen. This research has showed that in terms of evaluation APTI index of Platanus acerifolia is more tolerant and less sensitive to air pollution than Prunus subhirtella. The value of the APTI index at the Platanus acerifolia was 9,33, at the Prunus subhirtella APTI index was 7,84. During the evaluation of the maximum photochemical efficiency of photosystem II, statistically significant differences between the trees were not found. The values obtained via the method of chlorophyll fluorescence at both tree species were low, at the Platanus acerifolia it was 0,65, at the Prunus subhirtella it was 0,68. In terms of content of photosynthetic pigments, Prunus subhirtella has come out better. In almost all, it had greater content of pigments than the Platanus acerifolia. The results of the research were used in the draft for excursions for students of 7th grades of elementary schools to this area.
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The relationship of the selected pollutants in the air on the emergence of acute coronary syndrome in patients rescued by emergency medical service in Most region
Cmorej, Patrik Christian ; Nesvadba, Marcel (advisor) ; Dynáková, Šárka (referee)
Recent published studies have provided new information about the significant influence of environmental factors, especially air pollution in the development of acute coronary syndrome. Besides the well-known controllable and uncontrollable risk factors it is necessary to focus on non-conventional risk factors. The thesis aims to determine whether there is a statistically significant relationship between the days in which patients with acute coronary syndrome type STEMI were rescued by the EMS Most and the average daily concentrations of pollutants in air (PM2,5 and PM10, ozone, carbon monoxide and nitrogen dioxide) in seven previous days. The research methodology is based on a retrospective observational analysis of 116 patients with acute myocardial infarction in prehospital emergency care and the impact of the above pollutants in air with the use of statistical methods. The results in sets of ozone and nitric oxide effect presents a significant impact of day (p=0.0283/0.0424), sex (p=0.0216/0.0232) and age (p=0.0008/0 .0187). Although we have found a statistically significant effect of a day, gender and age in our analysis, it is necessary to continue research in a larger statistical sample to minimize the statistical errors. Key words: acute coronay syndrome, air pollution, emergency medical service
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Environment in China - current state, problems and perspectives
Salačová, Aneta ; De Castro, Tereza (advisor) ; Neumann, Pavel (referee)
The problematics of the environment has been underestimated in China for a long time. This country has been developing with incredible speed in the last three decades. The price for such growth is the current state of the environment in China. The thesis is divided into three chapters. The goal of this thesis is to evaluate the current state of the environment in China and the development of the stance of the Chinese government towards the protection of the environment in the new millennium. In connection with the economic growth is analysed the dependence on air pollution and Chinese GDP. Last but not least are offered possible solutions and perspectives of the future development of air pollution in China.
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Rating mass concentration of airborne dust in road transport depending on the speed of vehicles
PÍŠEK, David
This thesis focuses on an evaluation of the mass concentration of airborne dust in road transport in relation to the speed of the vehicles. Initially described are airborne dust, its types, fractions and impacts on humans and living environment. Furthermore, the work devotes itself to the measures taken against dustiness, the effects and impacts of transport on living environment and the closely related EURO European emission limits. Part of the work also includes a description of the DustTRAK II 8530 measuring device and individual measurements of dustiness of fraction size 10 m (PM10) during the movement of two vehicles of different weights on selected transport routes. The values measured and their analysis show that the size of the dust matter concentration in the atmosphere is affected by several factors. The main factors are the level and nature of pollution and the type of transport routes, the intensity of traffic, the speed of passing vehicles, the chosen track of passing vehicles in the traffic lane and the distribution of dirt on the road.
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