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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Genetic engineering of \kur{psbA} gene in \kur{Nicotiana tabacum}
 HUCKOVÁ, Dagmar
Transformation vector with mutated psbA gene and selective aadA gene was created and transferred into Nicotiana tabacum living cells using Biolistic bombardment. Due to homologous recombination, transformed plant lineage carrying D1-A209, D1-C-212 instead of D1-S209, D1-S212 in D1 protein in PS II was obtained. Seeds from transformed plant were harvested and homoplasmy of the first generation was tested. These mutations caused higher thermostability in Synechocystis sp. PCC6803 so the transformed plant is expected to be the first step in the study of PS II thermostability in higher plants.
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První údaj o resistenci Digitaria sanguinalis vůči atrazinu v České republice
 Salava, J. ; Chodová, D. ; Kočová, M. ; Krahulec, František
A survey on the occurrence of weeds surviving treatment with atrazine was carried out at the railways in the Czech Republic in 2000-05. Large crabgrass was widely distributed at the surveyed railway junctions. Seeds of large crabgrass were collected from plants growing in the surroundings of the railway station Prague-Bubny in 2003 and 2004. Resistance or susceptibility to atrazine was verified by the whole-plant response and chlorophyll fluorescence assays. A region of the psbA gene encoding D1 protein of Photosystem 2 was amplified by PCR and sequenced to determine the molecular basis for the atrazine resistance. It was found out that the resistance in the Prague-Bubny large crabgrass biotype was conferred by a glycine for serine substitution at residue 264 of the Dl protein of Photosystem 2. There was an excellent correspondence between the presence of the mutation and phenotypic resistance to atrazine of individual plants.
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