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Vliv konzumace stravy obohacené o pokrutiny z ostropestřce mariánského na hodnoty celkového bilirubinu, glukózy a triacylglycerolů
KOUBKOVÁ, Diana
The bachelor´s thesis on "The impact of consuming a diet enriched with milk thistle seeds on the levels of total bilirubin, glucose and triacylglycerols" focuses on the issue of liver diseases and possible subsequent treatment, with Silymarin primarily serving as a preventive measure against liver ailments. Nowadays, liver damage is becoming an increasingly common problem, potentially attributed to inappropriate dietary habits and an unhealthy lifestyle leading to a rising number of obese individuals. Excessive medication intake can also pose a risk. The effects of various hepatoprotective agents have been studied by scientists worldwide. The influence of Silymarin is relatively well-researched, with its hepatoprotective properties confirmed. The aim of this study was to determine whether this positive effect on the liver persists even when Silymarin seed powder is used in baking bread. In the research section of the Bachelor's thesis, blood samples from laboratory mice were obtained, and the serum was subjected to subsequent biochemical analysis. The data collected was processed into tables, calculating the average concentrations of total bilirubin, glucose, and triacylglycerols, along with standard deviations. Statistical evaluation was performed using ANOVA testing, determining the level of significance (p). It was found that paracetamol does not significantly alter the levels of total bilirubin and triacylglycerols, whereas a decrease in glucose levels was observed with paracetamol administration. When observing changes over the feeding period, total bilirubin remained largely unchanged, as did glucose levels. Triacylglycerol levels were higher in mice fed for 90 days compared to those fed for 14 days. The results of the Bachelor's thesis can serve as a source of data and be utilized for further research studies.
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Mesenchymal stem cells and their regenerative and immunomodulatory potential
Brychtová, Michaela ; Lysák, Daniel (advisor) ; Horák, Vratislav (referee) ; Raška, Milan (referee)
Mesenchymal stem cells and their regenerative and immunomodulatory potential Abstract Mesenchymal stem cells (MSCs) possess multidirectional regenerative ability, which, together with their immunomodulatory potential, makes them promising cell type for therapy of wide variety of diseases. Despite ongoing research, which proved MSCs application to be safe, reported effect of MSCs administration on patients is not convincingly beneficial yet. In our work we focused on elucidation of MSCs role in regeneration of vital organs, heart and liver, where a large damage is life threatening for patients and any improvement in therapy would save many lives. Similar situation is in Graft versus host disease (GVHD), where MSCs immunomodulatory properties could be beneficial. Role of MSCs in heart regeneration was examined in vitro. Primary adult swine cardiomyocytes (CMCs) were co-cultured with or without swine MSCs for 3 days and morphological and functional parameters (contractions, current, respiration) of CMCs were measured. MSCs showed supportive effect on CMCs survival, especially at day 3 of the experiment, where in co-culture was significantly higher number of viable CMCs with physiological morphology and maintained function. Effect of MSCs on liver regeneration was observed in swine model of chronic liver...
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Mesenchymal stem cells and their regenerative and immunomodulatory potential
Brychtová, Michaela ; Lysák, Daniel (advisor) ; Horák, Vratislav (referee) ; Raška, Milan (referee)
Mesenchymal stem cells and their regenerative and immunomodulatory potential Abstract Mesenchymal stem cells (MSCs) possess multidirectional regenerative ability, which, together with their immunomodulatory potential, makes them promising cell type for therapy of wide variety of diseases. Despite ongoing research, which proved MSCs application to be safe, reported effect of MSCs administration on patients is not convincingly beneficial yet. In our work we focused on elucidation of MSCs role in regeneration of vital organs, heart and liver, where a large damage is life threatening for patients and any improvement in therapy would save many lives. Similar situation is in Graft versus host disease (GVHD), where MSCs immunomodulatory properties could be beneficial. Role of MSCs in heart regeneration was examined in vitro. Primary adult swine cardiomyocytes (CMCs) were co-cultured with or without swine MSCs for 3 days and morphological and functional parameters (contractions, current, respiration) of CMCs were measured. MSCs showed supportive effect on CMCs survival, especially at day 3 of the experiment, where in co-culture was significantly higher number of viable CMCs with physiological morphology and maintained function. Effect of MSCs on liver regeneration was observed in swine model of chronic liver...
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