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Molekulární podmíněnost rezistence lnu vůči těžkým kovům a možnost jejího ovlivnění transgenozí
Vrbová, Miroslava
This work is focused on the molecular conditionality of flax resistance to heavy metals. Attention was therefore devoted to the identification of proteins whose role in stress response to heavy metals have not been discovered yet in flax. Two contrasting flax varieties displayed differences in steady state levels of short thiol peptides (GSH and PC) and heavy-metal binding proteins (ferritin and lipocalin) which can detoxify cadmium via chelation. These results are the first published analysis of the proteomic response to cadmium exposure in commercially important annual crop with potential use in phytoremediation. Another of the objectives was to verify the possibilities of influencing the flax resistance to heavy metal using transgenosis. There were obtained a multiplied flax plants, in which the presence of a transgene was verified, and these lines were tested for tolerance and accumulation of cadmium in both in vitro and in the field conditions.
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Preparation of rolls with addition of alternative flours
UHLÍŘOVÁ, Lenka
This bachelor thesis is focused on the production of ordinary pastry with the use of alternative flour. Four kinds of crop flour, made from seed pomace of flax (Linum usitatissimum L.), hemp (Cannabis sativa L.), squash (Cucurbita pepo L.) and milk thistle (Silybum marianum L.), were selected. In the literary part of the thesis, common pastry according to legislation, basic and auxiliary raw materials for production and production technology are all de-fined. The crops, from which the addition to wheat flour was processed, are also described. The practical part deals with the production of five types of wheat buns. Four of them are enriched with 5 % of flour made from seed pomace of the four mentioned crops. Production of buns was followed by sensory evaluation according to the descriptors. Weight lost by baking, the quantity of polyphenolic substances and antioxidant activity were also evaluated.
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Use of alternative plant flours for bread production
VÍTKOVÁ, Věra
This thesis is about importance of bread in diet, nutritional composition and the result of excessive at the human health. Part of the text deals with materials which are important section of research focused at enrichment of bread without negative effect on technical properties of dough. Is an effort to keep original sensory properties. Practical part deals with hemp, gourd, milk thistle and flax flours. This material was used in 5 or 10 % for baking 17 breads following the standard recipe. These breads were dried and grinded. Grounded breads were subjected to analysis of polyphenol content, antioxidant activity and nitrogen substance content. All breads containing oilseeds had better nutrition properties. There was not a problem with technological properties during dough processing.
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Production of enzyme hydrolysates from flaxseed proteins and evaluation of their properties
HAŠKOVÁ, Kateřina
This thesis focuses on effect on antioxidant properties and chosen functional properties: solubility, water and fat holding capacity, emulsification activity and stability caused by hydrolysis of protein isolates (PI) made from three different flax varieties (Libra, Agriol and Raciol). Part of this work was production of PI from flax seed. For hydrolysis trypsin, alcalase and papain were used - hydrolysis by trypsin was the mildest, while hydrolysis by papain was the most intensive one. Dynamics and degree of hydrolysis were also observed. Solubility of PI and hydrolysates was discovered to be very high (almost 100%). On the contrary water holding capacity was lower (3,93 g/g and less) than fat holding capacity (4,34 g/g), which increased upon hydrolysis for most enzymes, while water holding capacity decreased. Emulsification activity rised (1,47×) under the effect of hydrolysis, however stability dropped (1,3×). The best emulsifiction properties were found out in trypsin hydrolysates. Hydrolysis caused great impact on content of polyphenols which were in alcalase hydrolysates up to 3× higher and on antioxidant activity which grew in papain hydrolysates up to 2,5×.
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Využití lnu a lněné vlákniny v pekárenské výrobě
Chalupníková, Romana
The bachelor thesis describes the issue of growing flaxseed from soil preparation before sowing to harvest. Furthermore, it describes its qualitative factors and requirements that affect its quality. The paper describes the chemical composition of the seed, which has a high content of fiber, fats, proteins, etc. from the nutritional point of view and the use of flax seed in food. Flax seeds are used for the production of linseed oil, flax flour, etc., which are valuable for their high nutrient content. In the practical part is created a recipe with the addition of flax fiber from gold and brown flax. After the Rapid-Mix Test, the individual pieces of pastry are sensory and processed data.
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