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NG2-glia proliferation and differentiation following CNS injuries
Kirdajová, Denisa ; Anděrová, Miroslava (advisor) ; Procházka, Jan (referee) ; Novák, Ondřej (referee)
NG2-glia proliferation and differentiation following CNS injuries Abstract NG2 glia display wide proliferation and differentiation potential under physiological and pathological conditions. They are very well known as precursors of oligodendrocytes, however, following central nervous system (CNS) injury they play an important role in regeneration. For this reason, we examined these features following different types of brain disorders such as focal cerebral ischemia (FCI), cortical stab wound (SW), and demyelination (DEMY) in young (3-months-old) mice, in which NG2 glia are labeled by tdTomato under the Cspg4 promoter. In the case of FCI, the factor of age was also studied using 18-months-old mice. To address these issues, we employed many techniques on tissue/cellular levels, such as single-cell RT- qPCR, single-cell/bulk RNA-sequencing, immunohistochemistry, and the patch-clamp technique in situ. First, such approach enabled us to distinguish two main populations (NG2 glia, oligodendrocytes), each of them comprising four distinct subpopulations. Next, the expression profiling revealed that a subpopulation of NG2 glia expressing GFAP, a marker of reactive astrocytes, appears transiently after FCI. However, following less severe injury, namely the cortical SW and DEMY, subpopulations mirroring different...
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Case study of physiotherapy treatment of a patient with the diagnosis multiple sclerosis.
Valková, Barbora ; Novotná, Irena (advisor) ; Křížková, Štěpánka (referee)
Title: Case study of physiotherapy treatment of a patient with the diagnosis multiple sclerosis. Goals of assignment: Goal of common part of assignment was through study specialized literature regarding multiple sclerosis and it is etiology, prevalence of disease, diagnostic, types of treatment methods and procedures of physiotherapy. In special part was goal processing patient's casuistry his illness, application theoretical skills methods of physiotherapy and follow - up effect executed therapy. Methodology of assignment: Bachelor assignment is concentrated on physiotherapeutic treatment of patient with multiple sclerosis. In common part is described briefly anatomy and kinesiology which is pertinent to illness. In characterization of illness I deal with the probably etiology prevalence, individual types of illness, clinical image and factors which can affect illness. I devote my attention to diagnostic, prognosis and treatment. The treatment is divides into pharmacological, alternative and physiotherapeutic ones. The special part is written in the form of casuistry of patient with diagnosis multiple sclerosis. After the incoming kineziological assessment, long - term and short - term, therapeutic plan, process of single therapeutic units which include goal of the therapeutic unit, suggestion of...
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