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Biosensors based on modified nanoparticles
Mihajlović, Ana ; Majzlíková, Petra (referee) ; Hubálek, Jaromír (advisor)
This thesis deals with biosensors based on modified semiconductor quantum dots(AIIBIII). This work is divided into three main parts. The first deals with the theory necessary for the use of QDs in bioapplications, there are described methods of synthesis, application and bioconjugation of QDs . In the practical part, GSH-CdTe, CdTe-MPA and TGA-CdTe quantum dots were prepared by direct colloidal synthesis. These quantum dots were then modified by EDC in order to increase affinity to BSA (bovine serumalbumin). For the analysis of samples were used Infinite M200,Tecan and HORIBA Jobin Yvon FluoroLog detector Quanta . The third part is the summary of results and work achievements.
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Biofunctionalization of deposited quantum dots
Jakubechová, Jana ; Venerová, Tereza (referee) ; Drbohlavová, Jana (advisor)
The goal of this bachelor thesis is to functionalize the deposited quantum dots (QDs). QDs are prepared thanks to nanoporous alumina template in process of anodic oxidation of Ti film on a silicon wafer followed with modification of gold in process of pulse deposition. The final objective is the subsequent observation of fluorescent properties changes. QDs shows outstanding optical characteristics which can be used for labeling and imaging in biological systems. Thanks to their unique fotostability, they allow long term observation of biomolecules.
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Biofunctionalization of deposited quantum dots
Jakubechová, Jana ; Venerová, Tereza (referee) ; Drbohlavová, Jana (advisor)
The goal of this bachelor thesis is to functionalize the deposited quantum dots (QDs). QDs are prepared thanks to nanoporous alumina template in process of anodic oxidation of Ti film on a silicon wafer followed with modification of gold in process of pulse deposition. The final objective is the subsequent observation of fluorescent properties changes. QDs shows outstanding optical characteristics which can be used for labeling and imaging in biological systems. Thanks to their unique fotostability, they allow long term observation of biomolecules.
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Biosensors based on modified nanoparticles
Mihajlović, Ana ; Majzlíková, Petra (referee) ; Hubálek, Jaromír (advisor)
This thesis deals with biosensors based on modified semiconductor quantum dots(AIIBIII). This work is divided into three main parts. The first deals with the theory necessary for the use of QDs in bioapplications, there are described methods of synthesis, application and bioconjugation of QDs . In the practical part, GSH-CdTe, CdTe-MPA and TGA-CdTe quantum dots were prepared by direct colloidal synthesis. These quantum dots were then modified by EDC in order to increase affinity to BSA (bovine serumalbumin). For the analysis of samples were used Infinite M200,Tecan and HORIBA Jobin Yvon FluoroLog detector Quanta . The third part is the summary of results and work achievements.
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